Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles

Thermosensitive poly(N-isopropylacrylamide)-based polymer particles were synthesised, and screened for the adsorption of human immunoglobulin G (hIgG). At pH 9 the adsorption on microgel particles was strongly affected by temperature, approximately 40 mg hIgG/g support (90% of initial hIgG) being ad...

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Veröffentlicht in:Biotechnology letters 2006-12, Vol.28 (24), p.2019-2025
Hauptverfasser: Silva, Claudia S. O, Baptista, Ricardo P, Santos, Andrea M, Martinho, José M. G, Cabral, Joaquim M. S, Taipa, M. Ângela
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Sprache:eng
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Zusammenfassung:Thermosensitive poly(N-isopropylacrylamide)-based polymer particles were synthesised, and screened for the adsorption of human immunoglobulin G (hIgG). At pH 9 the adsorption on microgel particles was strongly affected by temperature, approximately 40 mg hIgG/g support (90% of initial hIgG) being adsorbed at 40°C but only 10% of initial hIgG at 25°C. At pH 5 the maximum adsorbed amount (20 mg hIgG/g support) was similar for both temperatures. The adsorption of hIgG on to charged poly(methyl methacrylate)/poly(N-isopropylacrylamide) core-shell latexes was negligible (5-10 mg hIgG/g support) at the same temperature and pH conditions. The lower adsorption of hIgG onto the core-shell particles is explained by steric interactions due to the small size of the shell.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-006-9188-2