Mechanism of Phage Mu-1 Integration: Nalidixic Acid Treatment Causes Clustering of Mu-1-Induced Mutations near Replication Origin

Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtai...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1978-10, Vol.75 (10), p.4940-4943
Hauptverfasser: Paolozzi, Luciano, Jucker, Richard, Calef, Enrico
Format: Artikel
Sprache:eng
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Zusammenfassung:Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtained from the control. Individual ratios of the frequency of mutants in the two cultures yield decreasing values from 6 to 1, starting from the point of origin of DNA replication to the termini of DNA replication. These results are compatible with the idea that Mu-1 integrates at the replication fork.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.75.10.4940