Mechanism of Phage Mu-1 Integration: Nalidixic Acid Treatment Causes Clustering of Mu-1-Induced Mutations near Replication Origin
Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtai...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1978-10, Vol.75 (10), p.4940-4943 |
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| container_issue | 10 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Paolozzi, Luciano Jucker, Richard Calef, Enrico |
| description | Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtained from the control. Individual ratios of the frequency of mutants in the two cultures yield decreasing values from 6 to 1, starting from the point of origin of DNA replication to the termini of DNA replication. These results are compatible with the idea that Mu-1 integrates at the replication fork. |
| doi_str_mv | 10.1073/pnas.75.10.4940 |
| format | Article |
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These results are compatible with the idea that Mu-1 integrates at the replication fork.</description><subject>Acid treatment</subject><subject>Bacteriophages</subject><subject>Coliphages - genetics</subject><subject>DNA</subject><subject>DNA Replication</subject><subject>DNA, Bacterial - metabolism</subject><subject>Genetic mutation</subject><subject>Infections</subject><subject>Lysogeny</subject><subject>Nalidixic Acid - pharmacology</subject><subject>Parasite hosts</subject><subject>Replication origin</subject><subject>Sugars</subject><subject>Virology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1v1DAQxS1EgW3hjIQQ8glO2dqOE8dIHKoVHyu1FKFytlxnnHWVOFvbQeXIf47TXRa4cLJm3u-Nx34IPadkSYkoT7dex6WocrHkkpMHaEGJpEXNJXmIFoQwUTSc8SfoOMYbQoisGvIYPWJNyQlfoJ8XYDbauzjg0eIvG90BvpgKitc-QRd0cqN_iz_r3rXuzhl8ZlyLrwLoNIBPeKWnCBGv-ikmCM5385TZX6x9Oxloc5Huh0TsQQf8Fba9M_cdfBlc5_xTdGR1H-HZ_jxB3z68v1p9Ks4vP65XZ-eF4TVJBWWVpVJYTbhuGwGyMtfCNJJDI1trqWD5xZpXvG6tFKIByN2yYdTm_2ANL0_Qu93c7XQ9QGvy-kH3ahvcoMMPNWqn_lW826hu_K7KsmalyP7Xe38YbyeISQ0uGuh77WGcohKcsapmJIOnO9CEMcYA9nAHJWrOTM2ZKVHN9ZxZdrz8e7UDvwspy2_28uz7Lf7xKzv1fYK7lMlX_yUz8GIH3MQ0hgNRS1KW5S-7dLVI</recordid><startdate>19781001</startdate><enddate>19781001</enddate><creator>Paolozzi, Luciano</creator><creator>Jucker, Richard</creator><creator>Calef, Enrico</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19781001</creationdate><title>Mechanism of Phage Mu-1 Integration: Nalidixic Acid Treatment Causes Clustering of Mu-1-Induced Mutations near Replication Origin</title><author>Paolozzi, Luciano ; Jucker, Richard ; Calef, Enrico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-125f197fa04ad87e95cb7c894e89dff172490a4546df9778eedff3821f1092843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Acid treatment</topic><topic>Bacteriophages</topic><topic>Coliphages - genetics</topic><topic>DNA</topic><topic>DNA Replication</topic><topic>DNA, Bacterial - metabolism</topic><topic>Genetic mutation</topic><topic>Infections</topic><topic>Lysogeny</topic><topic>Nalidixic Acid - pharmacology</topic><topic>Parasite hosts</topic><topic>Replication origin</topic><topic>Sugars</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paolozzi, Luciano</creatorcontrib><creatorcontrib>Jucker, Richard</creatorcontrib><creatorcontrib>Calef, Enrico</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paolozzi, Luciano</au><au>Jucker, Richard</au><au>Calef, Enrico</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of Phage Mu-1 Integration: Nalidixic Acid Treatment Causes Clustering of Mu-1-Induced Mutations near Replication Origin</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1978-10-01</date><risdate>1978</risdate><volume>75</volume><issue>10</issue><spage>4940</spage><epage>4943</epage><pages>4940-4943</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtained from the control. Individual ratios of the frequency of mutants in the two cultures yield decreasing values from 6 to 1, starting from the point of origin of DNA replication to the termini of DNA replication. These results are compatible with the idea that Mu-1 integrates at the replication fork.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>283404</pmid><doi>10.1073/pnas.75.10.4940</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1978-10, Vol.75 (10), p.4940-4943 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Acid treatment Bacteriophages Coliphages - genetics DNA DNA Replication DNA, Bacterial - metabolism Genetic mutation Infections Lysogeny Nalidixic Acid - pharmacology Parasite hosts Replication origin Sugars Virology |
| title | Mechanism of Phage Mu-1 Integration: Nalidixic Acid Treatment Causes Clustering of Mu-1-Induced Mutations near Replication Origin |
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