High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis
By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of h...
Gespeichert in:
Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2020-07, Vol.56 (54), p.743-746 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.
Undissociated binding of SSB to single-stranded DNA facilitates fast gel-free capillary electrophoresis analysis of fundamentally important DNA strand exchange reactions. |
---|---|
ISSN: | 1359-7345 1364-548X |
DOI: | 10.1039/d0cc02844d |