High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of h...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2020-07, Vol.56 (54), p.743-746
Hauptverfasser: Yu, Fangzhi, Yuan, Zheng, Zhang, Dapeng, Liu, Yan, Zhao, Qiang, Wang, Hailin
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Sprache:eng
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Zusammenfassung:By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error. Undissociated binding of SSB to single-stranded DNA facilitates fast gel-free capillary electrophoresis analysis of fundamentally important DNA strand exchange reactions.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc02844d