High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis
By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of h...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2020-07, Vol.56 (54), p.743-746 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Yu, Fangzhi Yuan, Zheng Zhang, Dapeng Liu, Yan Zhao, Qiang Wang, Hailin |
| description | By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.
Undissociated binding of SSB to single-stranded DNA facilitates fast gel-free capillary electrophoresis analysis of fundamentally important DNA strand exchange reactions. |
| doi_str_mv | 10.1039/d0cc02844d |
| format | Article |
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Undissociated binding of SSB to single-stranded DNA facilitates fast gel-free capillary electrophoresis analysis of fundamentally important DNA strand exchange reactions.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/d0cc02844d</identifier><identifier>PMID: 32514506</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Affinity ; Base Pair Mismatch ; Bioengineering ; Capillary electrophoresis ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - metabolism ; DNA, Single-Stranded - chemistry ; DNA, Single-Stranded - metabolism ; DNA-Binding Proteins - metabolism ; Electrophoresis ; Electrophoresis, Capillary - methods ; Escherichia coli - enzymology ; Escherichia coli Proteins - metabolism ; Exchanging ; Fluorescent Dyes - chemistry ; Kinetics ; Protein Binding ; Rec A Recombinases - metabolism</subject><ispartof>Chemical communications (Cambridge, England), 2020-07, Vol.56 (54), p.743-746</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-1a359d352a78156fdefffcb2afa0a07871fd1d02550142ca58f9f7ab6de358373</citedby><cites>FETCH-LOGICAL-c363t-1a359d352a78156fdefffcb2afa0a07871fd1d02550142ca58f9f7ab6de358373</cites><orcidid>0000-0001-7589-6727 ; 0000-0003-3448-8756 ; 0000-0002-1843-999X ; 0000-0001-5971-4858 ; 0000-0003-1676-9116 ; 0000-0002-4054-2803</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32514506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Fangzhi</creatorcontrib><creatorcontrib>Yuan, Zheng</creatorcontrib><creatorcontrib>Zhang, Dapeng</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Zhao, Qiang</creatorcontrib><creatorcontrib>Wang, Hailin</creatorcontrib><title>High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.
Undissociated binding of SSB to single-stranded DNA facilitates fast gel-free capillary electrophoresis analysis of fundamentally important DNA strand exchange reactions.</description><subject>Affinity</subject><subject>Base Pair Mismatch</subject><subject>Bioengineering</subject><subject>Capillary electrophoresis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>DNA, Single-Stranded - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Exchanging</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Kinetics</subject><subject>Protein Binding</subject><subject>Rec A Recombinases - metabolism</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1Lw0AQhhdRbK1evCsRb0J0P5P0WFK1QtGLgngJ0_1ot6RJ3E3A_nu3ttabc5mBeeZl5h2Ezgm-JZgN7xSWEtOMc3WA-oQlPBY8ez_c1GIYp4yLHjrxfolDEJEdox6jgnCBkz76mNj5IgZjbGXbdQSVirpKWe9raaHVKpLQ2LIEt450qWXr6mZRO-2tj0ztovHzKPKt24zpL7mAaq6DBpTrAJyiIwOl12e7PEBvD_ev-SSevjw-5aNpLFnC2phA2FIxQSHNiEiM0sYYOaNgAANOs5QYRRSmQmDCqQSRmaFJYZYozUTGUjZA11vdxtWfnfZtsaw7F5bwBeUUiyAduAG62VLS1d47bYrG2VW4qyC42NhYjHGe_9g4DvDlTrKbrbTao7--BeBqCzgv992_PxSNMoG5-I9h35BVgyU</recordid><startdate>20200711</startdate><enddate>20200711</enddate><creator>Yu, Fangzhi</creator><creator>Yuan, Zheng</creator><creator>Zhang, Dapeng</creator><creator>Liu, Yan</creator><creator>Zhao, Qiang</creator><creator>Wang, Hailin</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0001-7589-6727</orcidid><orcidid>https://orcid.org/0000-0003-3448-8756</orcidid><orcidid>https://orcid.org/0000-0002-1843-999X</orcidid><orcidid>https://orcid.org/0000-0001-5971-4858</orcidid><orcidid>https://orcid.org/0000-0003-1676-9116</orcidid><orcidid>https://orcid.org/0000-0002-4054-2803</orcidid></search><sort><creationdate>20200711</creationdate><title>High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis</title><author>Yu, Fangzhi ; Yuan, Zheng ; Zhang, Dapeng ; Liu, Yan ; Zhao, Qiang ; Wang, Hailin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-1a359d352a78156fdefffcb2afa0a07871fd1d02550142ca58f9f7ab6de358373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Affinity</topic><topic>Base Pair Mismatch</topic><topic>Bioengineering</topic><topic>Capillary electrophoresis</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - metabolism</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>DNA, Single-Stranded - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Exchanging</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Kinetics</topic><topic>Protein Binding</topic><topic>Rec A Recombinases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Fangzhi</creatorcontrib><creatorcontrib>Yuan, Zheng</creatorcontrib><creatorcontrib>Zhang, Dapeng</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Zhao, Qiang</creatorcontrib><creatorcontrib>Wang, Hailin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Fangzhi</au><au>Yuan, Zheng</au><au>Zhang, Dapeng</au><au>Liu, Yan</au><au>Zhao, Qiang</au><au>Wang, Hailin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2020-07-11</date><risdate>2020</risdate><volume>56</volume><issue>54</issue><spage>743</spage><epage>746</epage><pages>743-746</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.
Undissociated binding of SSB to single-stranded DNA facilitates fast gel-free capillary electrophoresis analysis of fundamentally important DNA strand exchange reactions.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>32514506</pmid><doi>10.1039/d0cc02844d</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0001-7589-6727</orcidid><orcidid>https://orcid.org/0000-0003-3448-8756</orcidid><orcidid>https://orcid.org/0000-0002-1843-999X</orcidid><orcidid>https://orcid.org/0000-0001-5971-4858</orcidid><orcidid>https://orcid.org/0000-0003-1676-9116</orcidid><orcidid>https://orcid.org/0000-0002-4054-2803</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2020-07, Vol.56 (54), p.743-746 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Affinity Base Pair Mismatch Bioengineering Capillary electrophoresis Deoxyribonucleic acid DNA DNA, Bacterial - metabolism DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism DNA-Binding Proteins - metabolism Electrophoresis Electrophoresis, Capillary - methods Escherichia coli - enzymology Escherichia coli Proteins - metabolism Exchanging Fluorescent Dyes - chemistry Kinetics Protein Binding Rec A Recombinases - metabolism |
| title | High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis |
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