High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis

By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of h...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2020-07, Vol.56 (54), p.743-746
Hauptverfasser:	Yu, Fangzhi, Yuan, Zheng, Zhang, Dapeng, Liu, Yan, Zhao, Qiang, Wang, Hailin
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Base Pair Mismatch Bioengineering Capillary electrophoresis Deoxyribonucleic acid DNA DNA, Bacterial - metabolism DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism DNA-Binding Proteins - metabolism Electrophoresis Electrophoresis, Capillary - methods Escherichia coli - enzymology Escherichia coli Proteins - metabolism Exchanging Fluorescent Dyes - chemistry Kinetics Protein Binding Rec A Recombinases - metabolism
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