Molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus

The invention provides a molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus. Genetic typing identification can be carried out on a PRRSV-1 genotype strain and a PRRSV-2 genotype strain through two groups of primer probes; through five g...

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Bibliographische Detailangaben
Hauptverfasser: TAO CHUNHAO, JIANG YITONG, JIA HONG, YUAN WEIFENG, ZHU HONGFEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus. Genetic typing identification can be carried out on a PRRSV-1 genotype strain and a PRRSV-2 genotype strain through two groups of primer probes; through five groups of primer probes, five kinds of PRRSV-2 strains of different pedigree lines can be further identified, and the five kinds of PRRSV-2 strains comprise an HP-PRRSV-like strain (Lineage 8.7), an NADC30-like strain (Lineage 1.8), an NADC34-like strain (Lineage 1.5), a QYYZ-like strain (Lineage 3) and a VR2332-like strain (Lineage 5). The primer probe combination provided by the invention can be used for identifying and diagnosing different genotypes of PRRSV and corresponding pedigree at one time through a fluorescent quantitative PCR (Polymerase Chain Reaction) method, has the advantages of accurate identification, high sensitivity, simplicity in operation, short time consumption and the like, and can provide an