Molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus

The invention provides a molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus. Genetic typing identification can be carried out on a PRRSV-1 genotype strain and a PRRSV-2 genotype strain through two groups of primer probes; through five g...

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Bibliographische Detailangaben
Hauptverfasser:	TAO CHUNHAO, JIANG YITONG, JIA HONG, YUAN WEIFENG, ZHU HONGFEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus. Genetic typing identification can be carried out on a PRRSV-1 genotype strain and a PRRSV-2 genotype strain through two groups of primer probes; through five groups of primer probes, five kinds of PRRSV-2 strains of different pedigree lines can be further identified, and the five kinds of PRRSV-2 strains comprise an HP-PRRSV-like strain (Lineage 8.7), an NADC30-like strain (Lineage 1.8), an NADC34-like strain (Lineage 1.5), a QYYZ-like strain (Lineage 3) and a VR2332-like strain (Lineage 5). The primer probe combination provided by the invention can be used for identifying and diagnosing different genotypes of PRRSV and corresponding pedigree at one time through a fluorescent quantitative PCR (Polymerase Chain Reaction) method, has the advantages of accurate identification, high sensitivity, simplicity in operation, short time consumption and the like, and can provide an