Molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus

The invention provides a molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus. Genetic typing identification can be carried out on a PRRSV-1 genotype strain and a PRRSV-2 genotype strain through two groups of primer probes; through five g...

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Hauptverfasser: TAO CHUNHAO, JIANG YITONG, JIA HONG, YUAN WEIFENG, ZHU HONGFEI
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creator TAO CHUNHAO
JIANG YITONG
JIA HONG
YUAN WEIFENG
ZHU HONGFEI
description The invention provides a molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus. Genetic typing identification can be carried out on a PRRSV-1 genotype strain and a PRRSV-2 genotype strain through two groups of primer probes; through five groups of primer probes, five kinds of PRRSV-2 strains of different pedigree lines can be further identified, and the five kinds of PRRSV-2 strains comprise an HP-PRRSV-like strain (Lineage 8.7), an NADC30-like strain (Lineage 1.8), an NADC34-like strain (Lineage 1.5), a QYYZ-like strain (Lineage 3) and a VR2332-like strain (Lineage 5). The primer probe combination provided by the invention can be used for identifying and diagnosing different genotypes of PRRSV and corresponding pedigree at one time through a fluorescent quantitative PCR (Polymerase Chain Reaction) method, has the advantages of accurate identification, high sensitivity, simplicity in operation, short time consumption and the like, and can provide an
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Molecular marker for genotyping and pedigree identification of porcine reproductive and respiratory syndrome virus
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