Molecular adaptation of Borrelia burgdorferi in the murine host

An analysis of expression of 137 lipoprotein genes on the course of murine infection revealed a two-step molecular adaptation by Borrelia burgdorferi, the Lyme disease spirochete. For the first step, regardless whether the initial inocula of B. burgdorferi expressed either all (cultured spirochetes)...

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Veröffentlicht in:The Journal of experimental medicine 2002-07, Vol.196 (2), p.275-280
Hauptverfasser: Liang, Fang Ting, Nelson, F Kenneth, Fikrig, Erol
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Nelson, F Kenneth
Fikrig, Erol
description An analysis of expression of 137 lipoprotein genes on the course of murine infection revealed a two-step molecular adaptation by Borrelia burgdorferi, the Lyme disease spirochete. For the first step, regardless whether the initial inocula of B. burgdorferi expressed either all (cultured spirochetes) or less than 40 (host-adapted spirochetes) of the 137 lipoprotein genes, the spirochetes were modulated to transcribe 116 of the genes within 10 d after being introduced to the murine host. This step of adaptation was induced by the microenvironment of the host tissue. During the second step, which was forced by host immune selection pressure and occurred between 17 and 30 d after infection, B. burgdorferi down-regulated most of the lipoprotein genes and expressed less than 40 of the 137 genes. This novel adaptation mechanism could be a critical step for B. burgdorferi to proceed to chronic infection, as the pathogen would be cleared at the early stage of infection if the spirochetes failed to undergo this process.
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subjects Adaptation, Physiological
Animals
Antibodies, Bacterial - biosynthesis
Arachnid Vectors - microbiology
Bacterial Proteins - genetics
Borrelia burgdorferi - genetics
Borrelia burgdorferi - growth & development
Borrelia burgdorferi - immunology
Borrelia burgdorferi - physiology
Brief Definitive Report
Gene Expression Profiling
Genes, Bacterial
Lipoproteins - genetics
Lyme Disease - immunology
Lyme Disease - microbiology
Mice
Mice, Inbred C3H
Mice, SCID
Oligonucleotide Array Sequence Analysis
Ticks - microbiology
title Molecular adaptation of Borrelia burgdorferi in the murine host
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