The raising and characterization of cortisol-binding antiserum

Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The spec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Clinica chimica acta 1975-01, Vol.58 (2), p.173-176
Hauptverfasser: Brombacher, P.J., Gijzen, A.H.J., Janssens, H.H.P., Soons, M.P.J.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 176
container_issue 2
container_start_page 173
container_title Clinica chimica acta
container_volume 58
creator Brombacher, P.J.
Gijzen, A.H.J.
Janssens, H.H.P.
Soons, M.P.J.
description Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]
doi_str_mv 10.1016/S0009-8981(75)80009-X
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82786815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S000989817580009X</els_id><sourcerecordid>82786815</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-d1621c3cb560c023253375c2f737c034dc2603d246c38a7b594b118a2a9067fe3</originalsourceid><addsrcrecordid>eNqFkEtLw0AUhQdRan38hEJWoovoPDKPbBQpvqDgwgrdDZM7EzuSJnUmEfTXmzZFl64uh3POvdwPoQnBlwQTcfWCMc5TlStyLvmF2qrFHhoTJVnKspzuo_Fv5BAdxfjeywwLMkIjQigVGR6j6_nSJcH46Ou3xNQ2gaUJBloX_LdpfVMnTZlAE1ofmyotfG2HYK9d6FYn6KA0VXSnu3mMXu_v5tPHdPb88DS9naXABG5TSwQlwKDgAgOmjHLGJAdaSiYBs8wCFZhZmglgysiC51lBiDLU5FjI0rFjdDbsXYfmo3Ox1SsfwVWVqV3TRa2oVEIR3gf5EITQxBhcqdfBr0z40gTrDTe95aY3ULTkestNL_reZHegK1bO_rUGUL1_M_iu__LTu6AjeFeDsz44aLVt_D8XfgDhbnwk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82786815</pqid></control><display><type>article</type><title>The raising and characterization of cortisol-binding antiserum</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Brombacher, P.J. ; Gijzen, A.H.J. ; Janssens, H.H.P. ; Soons, M.P.J.</creator><creatorcontrib>Brombacher, P.J. ; Gijzen, A.H.J. ; Janssens, H.H.P. ; Soons, M.P.J.</creatorcontrib><description>Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(75)80009-X</identifier><identifier>PMID: 1122640</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibody Formation ; Binding Sites, Antibody ; Chromatography, Gel ; Hydrocortisone - immunology ; Immune Sera ; Iodine Radioisotopes ; Methods ; Protein Binding ; Rabbits - immunology ; Radioimmunoassay ; Transcortin</subject><ispartof>Clinica chimica acta, 1975-01, Vol.58 (2), p.173-176</ispartof><rights>1975 Elsevier Scientific Publishing Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-d1621c3cb560c023253375c2f737c034dc2603d246c38a7b594b118a2a9067fe3</citedby><cites>FETCH-LOGICAL-c360t-d1621c3cb560c023253375c2f737c034dc2603d246c38a7b594b118a2a9067fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-8981(75)80009-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1122640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brombacher, P.J.</creatorcontrib><creatorcontrib>Gijzen, A.H.J.</creatorcontrib><creatorcontrib>Janssens, H.H.P.</creatorcontrib><creatorcontrib>Soons, M.P.J.</creatorcontrib><title>The raising and characterization of cortisol-binding antiserum</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Binding Sites, Antibody</subject><subject>Chromatography, Gel</subject><subject>Hydrocortisone - immunology</subject><subject>Immune Sera</subject><subject>Iodine Radioisotopes</subject><subject>Methods</subject><subject>Protein Binding</subject><subject>Rabbits - immunology</subject><subject>Radioimmunoassay</subject><subject>Transcortin</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLw0AUhQdRan38hEJWoovoPDKPbBQpvqDgwgrdDZM7EzuSJnUmEfTXmzZFl64uh3POvdwPoQnBlwQTcfWCMc5TlStyLvmF2qrFHhoTJVnKspzuo_Fv5BAdxfjeywwLMkIjQigVGR6j6_nSJcH46Ou3xNQ2gaUJBloX_LdpfVMnTZlAE1ofmyotfG2HYK9d6FYn6KA0VXSnu3mMXu_v5tPHdPb88DS9naXABG5TSwQlwKDgAgOmjHLGJAdaSiYBs8wCFZhZmglgysiC51lBiDLU5FjI0rFjdDbsXYfmo3Ox1SsfwVWVqV3TRa2oVEIR3gf5EITQxBhcqdfBr0z40gTrDTe95aY3ULTkestNL_reZHegK1bO_rUGUL1_M_iu__LTu6AjeFeDsz44aLVt_D8XfgDhbnwk</recordid><startdate>19750101</startdate><enddate>19750101</enddate><creator>Brombacher, P.J.</creator><creator>Gijzen, A.H.J.</creator><creator>Janssens, H.H.P.</creator><creator>Soons, M.P.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750101</creationdate><title>The raising and characterization of cortisol-binding antiserum</title><author>Brombacher, P.J. ; Gijzen, A.H.J. ; Janssens, H.H.P. ; Soons, M.P.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-d1621c3cb560c023253375c2f737c034dc2603d246c38a7b594b118a2a9067fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Binding Sites, Antibody</topic><topic>Chromatography, Gel</topic><topic>Hydrocortisone - immunology</topic><topic>Immune Sera</topic><topic>Iodine Radioisotopes</topic><topic>Methods</topic><topic>Protein Binding</topic><topic>Rabbits - immunology</topic><topic>Radioimmunoassay</topic><topic>Transcortin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brombacher, P.J.</creatorcontrib><creatorcontrib>Gijzen, A.H.J.</creatorcontrib><creatorcontrib>Janssens, H.H.P.</creatorcontrib><creatorcontrib>Soons, M.P.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brombacher, P.J.</au><au>Gijzen, A.H.J.</au><au>Janssens, H.H.P.</au><au>Soons, M.P.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The raising and characterization of cortisol-binding antiserum</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1975-01-01</date><risdate>1975</risdate><volume>58</volume><issue>2</issue><spage>173</spage><epage>176</epage><pages>173-176</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>1122640</pmid><doi>10.1016/S0009-8981(75)80009-X</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0009-8981
ispartof Clinica chimica acta, 1975-01, Vol.58 (2), p.173-176
issn 0009-8981
1873-3492
language eng
recordid cdi_proquest_miscellaneous_82786815
source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Animals
Antibody Formation
Binding Sites, Antibody
Chromatography, Gel
Hydrocortisone - immunology
Immune Sera
Iodine Radioisotopes
Methods
Protein Binding
Rabbits - immunology
Radioimmunoassay
Transcortin
title The raising and characterization of cortisol-binding antiserum
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T12%3A03%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20raising%20and%20characterization%20of%20cortisol-binding%20antiserum&rft.jtitle=Clinica%20chimica%20acta&rft.au=Brombacher,%20P.J.&rft.date=1975-01-01&rft.volume=58&rft.issue=2&rft.spage=173&rft.epage=176&rft.pages=173-176&rft.issn=0009-8981&rft.eissn=1873-3492&rft_id=info:doi/10.1016/S0009-8981(75)80009-X&rft_dat=%3Cproquest_cross%3E82786815%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82786815&rft_id=info:pmid/1122640&rft_els_id=S000989817580009X&rfr_iscdi=true