The raising and characterization of cortisol-binding antiserum
Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The spec...
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Veröffentlicht in: | Clinica chimica acta 1975-01, Vol.58 (2), p.173-176 |
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container_title | Clinica chimica acta |
container_volume | 58 |
creator | Brombacher, P.J. Gijzen, A.H.J. Janssens, H.H.P. Soons, M.P.J. |
description | Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail.
The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such.
The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ] |
doi_str_mv | 10.1016/S0009-8981(75)80009-X |
format | Article |
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The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such.
The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(75)80009-X</identifier><identifier>PMID: 1122640</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibody Formation ; Binding Sites, Antibody ; Chromatography, Gel ; Hydrocortisone - immunology ; Immune Sera ; Iodine Radioisotopes ; Methods ; Protein Binding ; Rabbits - immunology ; Radioimmunoassay ; Transcortin</subject><ispartof>Clinica chimica acta, 1975-01, Vol.58 (2), p.173-176</ispartof><rights>1975 Elsevier Scientific Publishing Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-d1621c3cb560c023253375c2f737c034dc2603d246c38a7b594b118a2a9067fe3</citedby><cites>FETCH-LOGICAL-c360t-d1621c3cb560c023253375c2f737c034dc2603d246c38a7b594b118a2a9067fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-8981(75)80009-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1122640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brombacher, P.J.</creatorcontrib><creatorcontrib>Gijzen, A.H.J.</creatorcontrib><creatorcontrib>Janssens, H.H.P.</creatorcontrib><creatorcontrib>Soons, M.P.J.</creatorcontrib><title>The raising and characterization of cortisol-binding antiserum</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail.
The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such.
The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Binding Sites, Antibody</subject><subject>Chromatography, Gel</subject><subject>Hydrocortisone - immunology</subject><subject>Immune Sera</subject><subject>Iodine Radioisotopes</subject><subject>Methods</subject><subject>Protein Binding</subject><subject>Rabbits - immunology</subject><subject>Radioimmunoassay</subject><subject>Transcortin</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLw0AUhQdRan38hEJWoovoPDKPbBQpvqDgwgrdDZM7EzuSJnUmEfTXmzZFl64uh3POvdwPoQnBlwQTcfWCMc5TlStyLvmF2qrFHhoTJVnKspzuo_Fv5BAdxfjeywwLMkIjQigVGR6j6_nSJcH46Ou3xNQ2gaUJBloX_LdpfVMnTZlAE1ofmyotfG2HYK9d6FYn6KA0VXSnu3mMXu_v5tPHdPb88DS9naXABG5TSwQlwKDgAgOmjHLGJAdaSiYBs8wCFZhZmglgysiC51lBiDLU5FjI0rFjdDbsXYfmo3Ox1SsfwVWVqV3TRa2oVEIR3gf5EITQxBhcqdfBr0z40gTrDTe95aY3ULTkestNL_reZHegK1bO_rUGUL1_M_iu__LTu6AjeFeDsz44aLVt_D8XfgDhbnwk</recordid><startdate>19750101</startdate><enddate>19750101</enddate><creator>Brombacher, P.J.</creator><creator>Gijzen, A.H.J.</creator><creator>Janssens, H.H.P.</creator><creator>Soons, M.P.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750101</creationdate><title>The raising and characterization of cortisol-binding antiserum</title><author>Brombacher, P.J. ; Gijzen, A.H.J. ; Janssens, H.H.P. ; Soons, M.P.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-d1621c3cb560c023253375c2f737c034dc2603d246c38a7b594b118a2a9067fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Binding Sites, Antibody</topic><topic>Chromatography, Gel</topic><topic>Hydrocortisone - immunology</topic><topic>Immune Sera</topic><topic>Iodine Radioisotopes</topic><topic>Methods</topic><topic>Protein Binding</topic><topic>Rabbits - immunology</topic><topic>Radioimmunoassay</topic><topic>Transcortin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brombacher, P.J.</creatorcontrib><creatorcontrib>Gijzen, A.H.J.</creatorcontrib><creatorcontrib>Janssens, H.H.P.</creatorcontrib><creatorcontrib>Soons, M.P.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brombacher, P.J.</au><au>Gijzen, A.H.J.</au><au>Janssens, H.H.P.</au><au>Soons, M.P.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The raising and characterization of cortisol-binding antiserum</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1975-01-01</date><risdate>1975</risdate><volume>58</volume><issue>2</issue><spage>173</spage><epage>176</epage><pages>173-176</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail.
The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such.
The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>1122640</pmid><doi>10.1016/S0009-8981(75)80009-X</doi><tpages>4</tpages></addata></record> |
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subjects | Animals Antibody Formation Binding Sites, Antibody Chromatography, Gel Hydrocortisone - immunology Immune Sera Iodine Radioisotopes Methods Protein Binding Rabbits - immunology Radioimmunoassay Transcortin |
title | The raising and characterization of cortisol-binding antiserum |
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