The raising and characterization of cortisol-binding antiserum

The raising and characterization of cortisol-binding antiserum

Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The spec...

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Veröffentlicht in:Clinica chimica acta 1975-01, Vol.58 (2), p.173-176
Hauptverfasser: Brombacher, P.J., Gijzen, A.H.J., Janssens, H.H.P., Soons, M.P.J.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibody Formation
Binding Sites, Antibody
Chromatography, Gel
Hydrocortisone - immunology
Immune Sera
Iodine Radioisotopes
Methods
Protein Binding
Rabbits - immunology
Radioimmunoassay
Transcortin
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Zusammenfassung:Synthesis of a cortisol-albumin compound and the raising of antibodies against cortisol in rabbits are described in detail. The steroid-protein complex is characterized by a number of reactions regarding the amount of cortisol coupled and the ability of the coupled steroid to react as such. The specificity of the harvested antiserum and transcortin towards the coupling of several related steroids are compared; the rabbit antiserum appears to be the more selective binding agent. [t ]
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(75)80009-X