Metal-Chelate Affinity Chromatography as a Separation Tool
In this paper, the authors compare the agarose-based metal chelate adsorbents with those of silica-based ones under similar aqueous elution conditions, in order to design scaled-up metal-chelate chromatographic procedures for active proteins and peptide purification. Synthetic nucleotides and dipept...
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Veröffentlicht in: | Annals of the New York Academy of Sciences 1983-01, Vol.413 (1), p.300-306 |
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creator | FANOU-AYI, L. VIJAYALAKSHMI, M. |
description | In this paper, the authors compare the agarose-based metal chelate adsorbents with those of silica-based ones under similar aqueous elution conditions, in order to design scaled-up metal-chelate chromatographic procedures for active proteins and peptide purification. Synthetic nucleotides and dipeptides are used as model substances to better elucidate the mechanism involved. |
doi_str_mv | 10.1111/j.1749-6632.1983.tb47904.x |
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Psychology</topic><topic>Metals</topic><topic>Methods. Procedures. 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subjects | affinity chromatography Animals Biological and medical sciences Biotechnology Cattle chelates Chelating Agents Chromatography, Affinity - methods Dipeptides - isolation & purification Female Fundamental and applied biological sciences. Psychology Metals Methods. Procedures. Technologies Milk Proteins - isolation & purification Oxytocin - isolation & purification proteins Proteins - isolation & purification Ribonucleotides - isolation & purification Structure-Activity Relationship transition metals Various methods and equipments Vasopressins - isolation & purification |
title | Metal-Chelate Affinity Chromatography as a Separation Tool |
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