Metal-Chelate Affinity Chromatography as a Separation Tool

In this paper, the authors compare the agarose-based metal chelate adsorbents with those of silica-based ones under similar aqueous elution conditions, in order to design scaled-up metal-chelate chromatographic procedures for active proteins and peptide purification. Synthetic nucleotides and dipept...

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Veröffentlicht in:	Annals of the New York Academy of Sciences 1983-01, Vol.413 (1), p.300-306
Hauptverfasser:	FANOU-AYI, L., VIJAYALAKSHMI, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity chromatography Animals Biological and medical sciences Biotechnology Cattle chelates Chelating Agents Chromatography, Affinity - methods Dipeptides - isolation & purification Female Fundamental and applied biological sciences. Psychology Metals Methods. Procedures. Technologies Milk Proteins - isolation & purification Oxytocin - isolation & purification proteins Proteins - isolation & purification Ribonucleotides - isolation & purification Structure-Activity Relationship transition metals Various methods and equipments Vasopressins - isolation & purification
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Beschreibung
Zusammenfassung:	In this paper, the authors compare the agarose-based metal chelate adsorbents with those of silica-based ones under similar aqueous elution conditions, in order to design scaled-up metal-chelate chromatographic procedures for active proteins and peptide purification. Synthetic nucleotides and dipeptides are used as model substances to better elucidate the mechanism involved.
ISSN:	0077-8923 1749-6632
DOI:	10.1111/j.1749-6632.1983.tb47904.x