Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation

A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA—reversibly precipitated with the cationic detergent cetylpyridinium bromide—can be subjected to further enzymatic manipulations within minutes. This method is a...

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Veröffentlicht in:Analytical biochemistry 1983-01, Vol.135 (2), p.264-268
Hauptverfasser: Geck, P., Nász, I.
Format: Artikel
Sprache:eng
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Zusammenfassung:A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA—reversibly precipitated with the cationic detergent cetylpyridinium bromide—can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(83)90681-4