Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation
A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA—reversibly precipitated with the cationic detergent cetylpyridinium bromide—can be subjected to further enzymatic manipulations within minutes. This method is a...
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| Veröffentlicht in: | Analytical biochemistry 1983-01, Vol.135 (2), p.264-268 |
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| container_end_page | 268 |
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| container_title | Analytical biochemistry |
| container_volume | 135 |
| creator | Geck, P. Nász, I. |
| description | A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA—reversibly precipitated with the cationic detergent cetylpyridinium bromide—can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA. |
| doi_str_mv | 10.1016/0003-2697(83)90681-4 |
| format | Article |
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The DNA—reversibly precipitated with the cationic detergent cetylpyridinium bromide—can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(83)90681-4</identifier><identifier>PMID: 6660501</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; cationic detergent ; Cetylpyridinium ; Chemical Phenomena ; Chemical Precipitation ; Chemistry ; Chromatography - methods ; DNA - isolation & purification ; DNA purification ; Dna, deoxyribonucleoproteins ; Fundamental and applied biological sciences. Psychology ; Hydroxyapatites ; hydroxylapatite chromatography ; Nucleic acids ; Phosphates ; plasmid preparation ; Plasmids ; Pyridinium Compounds ; restriction enzymes</subject><ispartof>Analytical biochemistry, 1983-01, Vol.135 (2), p.264-268</ispartof><rights>1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-1acca93d46b29c81132deb7d18cdcb9a991d67545c556a7b4b685ddd285d7fc53</citedby><cites>FETCH-LOGICAL-c417t-1acca93d46b29c81132deb7d18cdcb9a991d67545c556a7b4b685ddd285d7fc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(83)90681-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9575335$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6660501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geck, P.</creatorcontrib><creatorcontrib>Nász, I.</creatorcontrib><title>Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA—reversibly precipitated with the cationic detergent cetylpyridinium bromide—can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>cationic detergent</subject><subject>Cetylpyridinium</subject><subject>Chemical Phenomena</subject><subject>Chemical Precipitation</subject><subject>Chemistry</subject><subject>Chromatography - methods</subject><subject>DNA - isolation & purification</subject><subject>DNA purification</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydroxyapatites</subject><subject>hydroxylapatite chromatography</subject><subject>Nucleic acids</subject><subject>Phosphates</subject><subject>plasmid preparation</subject><subject>Plasmids</subject><subject>Pyridinium Compounds</subject><subject>restriction enzymes</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxa0KVLal36CVfKhQkQjYcezYF6Rq-StVcKFny7EnXaMkTm0vkG-Pt7vaI1xmDvN7o5n3ELqk5C0lVLwjhLCqFqq9key1IkLSqjlBK0qUqAgj6hlaHZEX6Cyln4RQ2nBxik6FEIQTukLDOkwWphxNBvcGO_8AKftuAPzh2y02fYaIN4uL4c8ymNlknwHbTQyjyeEhmnmz4N8-b7CFvAzzEr3zk9-OuCuId4DnCNbPPhdlmF6i570ZElwc-jm6__Txx_pLdff989f17V1lG9rmihprjWKuEV2trKSU1Q661lFpne2UUYo60fKGW86FabumE5I75-pS295ydo5e7ffOMTxuy0N69MnCMJgJwjZpSSSrOVP_BSmTRBBZF7DZgzaGlCL0eo5-NHHRlOhdGnpntd5ZrSXTT2nopsiuDvu33QjuKDrYX-bXh7lJ1gx9NJP16Ygp3nLGdv-832NQTPvlIepkPZTcnC_2Zu2C__cdfwHKj6iU</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Geck, P.</creator><creator>Nász, I.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation</title><author>Geck, P. ; Nász, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-1acca93d46b29c81132deb7d18cdcb9a991d67545c556a7b4b685ddd285d7fc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>cationic detergent</topic><topic>Cetylpyridinium</topic><topic>Chemical Phenomena</topic><topic>Chemical Precipitation</topic><topic>Chemistry</topic><topic>Chromatography - methods</topic><topic>DNA - isolation & purification</topic><topic>DNA purification</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydroxyapatites</topic><topic>hydroxylapatite chromatography</topic><topic>Nucleic acids</topic><topic>Phosphates</topic><topic>plasmid preparation</topic><topic>Plasmids</topic><topic>Pyridinium Compounds</topic><topic>restriction enzymes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Geck, P.</creatorcontrib><creatorcontrib>Nász, I.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geck, P.</au><au>Nász, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>135</volume><issue>2</issue><spage>264</spage><epage>268</epage><pages>264-268</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA—reversibly precipitated with the cationic detergent cetylpyridinium bromide—can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>6660501</pmid><doi>10.1016/0003-2697(83)90681-4</doi><tpages>5</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1983-01, Vol.135 (2), p.264-268 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences cationic detergent Cetylpyridinium Chemical Phenomena Chemical Precipitation Chemistry Chromatography - methods DNA - isolation & purification DNA purification Dna, deoxyribonucleoproteins Fundamental and applied biological sciences. Psychology Hydroxyapatites hydroxylapatite chromatography Nucleic acids Phosphates plasmid preparation Plasmids Pyridinium Compounds restriction enzymes |
| title | Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation |
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