Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa

A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]L...

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Veröffentlicht in:FEBS letters 1986-11, Vol.208 (1), p.26-30
Hauptverfasser: HOPPE, U, HOPPE, E. M, PESKAR, B. M, PESKAR, B. A
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container_start_page 26
container_title FEBS letters
container_volume 208
creator HOPPE, U
HOPPE, E. M
PESKAR, B. M
PESKAR, B. A
description A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material.
doi_str_mv 10.1016/0014-5793(86)81524-1
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A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1986-11-10</date><risdate>1986</risdate><volume>208</volume><issue>1</issue><spage>26</spage><epage>30</epage><pages>26-30</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>3021536</pmid><doi>10.1016/0014-5793(86)81524-1</doi><tpages>5</tpages></addata></record>
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source MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Amino Acids, Sulfur - metabolism
Animals
Applied sciences
Biological and medical sciences
Digestive system
Exact sciences and technology
Gastric Mucosa - metabolism
In Vitro Techniques
Leukotriene E4
Male
Masoprocol - pharmacology
Medical sciences
Other techniques and industries
Pharmacology. Drug treatments
Radioimmunoassay
Rats
Rats, Inbred Strains
SRS-A - analogs & derivatives
SRS-A - analysis
title Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa
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