Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa
A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]L...
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Veröffentlicht in: | FEBS letters 1986-11, Vol.208 (1), p.26-30 |
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creator | HOPPE, U HOPPE, E. M PESKAR, B. M PESKAR, B. A |
description | A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material. |
doi_str_mv | 10.1016/0014-5793(86)81524-1 |
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It could be demonstrated that this method is suitable for determination of SP-LT in biological material.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(86)81524-1</identifier><identifier>PMID: 3021536</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Amino Acids, Sulfur - metabolism ; Animals ; Applied sciences ; Biological and medical sciences ; Digestive system ; Exact sciences and technology ; Gastric Mucosa - metabolism ; In Vitro Techniques ; Leukotriene E4 ; Male ; Masoprocol - pharmacology ; Medical sciences ; Other techniques and industries ; Pharmacology. 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M</creatorcontrib><creatorcontrib>PESKAR, B. M</creatorcontrib><creatorcontrib>PESKAR, B. A</creatorcontrib><title>Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material.</description><subject>Amino Acids, Sulfur - metabolism</subject><subject>Animals</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Digestive system</subject><subject>Exact sciences and technology</subject><subject>Gastric Mucosa - metabolism</subject><subject>In Vitro Techniques</subject><subject>Leukotriene E4</subject><subject>Male</subject><subject>Masoprocol - pharmacology</subject><subject>Medical sciences</subject><subject>Other techniques and industries</subject><subject>Pharmacology. Drug treatments</subject><subject>Radioimmunoassay</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>SRS-A - analogs & derivatives</subject><subject>SRS-A - analysis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtL3kAUhgdp0c_LP1CYRSm6SJ1rMtNdEbUFoSC6DieTkzJtkknnsnDjb2-0Qbrr6nDO87xn8RJyytknznh9yRhXlW6sPDf1heFaqIrvkR03jaykqs07sntTDshhSj_Zuhtu98m-ZIJrWe_I8z30PvhpKnOAlOCJDiHSEcuvkKPHGem1-kxLwtd7jxnj5GfIPsw0DDSHDCNNZRx8HxZcsu-x-jcdcUR4Sccw0QiZ_oC0Iken4kKCY_J-gDHhyTaPyOPN9cPV1-ru--23qy931SJqlavGaic17xSI3kglGm2Z4wy06p3sjOOdVd1geSMEarQdaOxEr6WSqBzwQR6Rj3__LjH8LphyO_nkcBxhxlBS2zRrpZyLVTzbxNJN2LdL9BPEp3YrbOUfNg7JwThEmJ1Pb5qRWllm_q8xo62RfwDMhomu</recordid><startdate>19861110</startdate><enddate>19861110</enddate><creator>HOPPE, U</creator><creator>HOPPE, E. M</creator><creator>PESKAR, B. M</creator><creator>PESKAR, B. A</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19861110</creationdate><title>Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa</title><author>HOPPE, U ; HOPPE, E. M ; PESKAR, B. M ; PESKAR, B. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p264t-795c351b4a2d83427590c10a54dc3b8c1b94bf91722e5e9ba5eb2d5343e4ca1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Amino Acids, Sulfur - metabolism</topic><topic>Animals</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Digestive system</topic><topic>Exact sciences and technology</topic><topic>Gastric Mucosa - metabolism</topic><topic>In Vitro Techniques</topic><topic>Leukotriene E4</topic><topic>Male</topic><topic>Masoprocol - pharmacology</topic><topic>Medical sciences</topic><topic>Other techniques and industries</topic><topic>Pharmacology. Drug treatments</topic><topic>Radioimmunoassay</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>SRS-A - analogs & derivatives</topic><topic>SRS-A - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HOPPE, U</creatorcontrib><creatorcontrib>HOPPE, E. M</creatorcontrib><creatorcontrib>PESKAR, B. M</creatorcontrib><creatorcontrib>PESKAR, B. A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HOPPE, U</au><au>HOPPE, E. M</au><au>PESKAR, B. M</au><au>PESKAR, B. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1986-11-10</date><risdate>1986</risdate><volume>208</volume><issue>1</issue><spage>26</spage><epage>30</epage><pages>26-30</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>3021536</pmid><doi>10.1016/0014-5793(86)81524-1</doi><tpages>5</tpages></addata></record> |
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subjects | Amino Acids, Sulfur - metabolism Animals Applied sciences Biological and medical sciences Digestive system Exact sciences and technology Gastric Mucosa - metabolism In Vitro Techniques Leukotriene E4 Male Masoprocol - pharmacology Medical sciences Other techniques and industries Pharmacology. Drug treatments Radioimmunoassay Rats Rats, Inbred Strains SRS-A - analogs & derivatives SRS-A - analysis |
title | Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa |
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