Radioimmunoassay for leukotriene E4: use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa

A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]L...

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Veröffentlicht in:	FEBS letters 1986-11, Vol.208 (1), p.26-30
Hauptverfasser:	HOPPE, U, HOPPE, E. M, PESKAR, B. M, PESKAR, B. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids, Sulfur - metabolism Animals Applied sciences Biological and medical sciences Digestive system Exact sciences and technology Gastric Mucosa - metabolism In Vitro Techniques Leukotriene E4 Male Masoprocol - pharmacology Medical sciences Other techniques and industries Pharmacology. Drug treatments Radioimmunoassay Rats Rats, Inbred Strains SRS-A - analogs & derivatives SRS-A - analysis
Online-Zugang:	Volltext
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