Sickle Cell Disease of Transgenic SAD Mice

Erythrocyte sickling on deoxygenation in vitro occurs in transgenic SAD mice, hemizygous for a modified human sickle hemoglobin, HbSAD [α2β2S(β6val)Antilles(β23lle)D-Punjab(β121Gln)] (SAD-1, 19% HbSAD; β-thal/SAD-1, 26% HbSAD). The present study examines the cellular defects in vivo and pathologic c...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Blood 1994-11, Vol.84 (9), p.3189-3197
Hauptverfasser: Trudel, Marie, De Paepe, Monique E., Chrέtien, Nathalie, Saadane, Nacέra, Jacmain, Janik, Sorette, Martin, Hoang, Trang, Beuzard, Yves
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Erythrocyte sickling on deoxygenation in vitro occurs in transgenic SAD mice, hemizygous for a modified human sickle hemoglobin, HbSAD [α2β2S(β6val)Antilles(β23lle)D-Punjab(β121Gln)] (SAD-1, 19% HbSAD; β-thal/SAD-1, 26% HbSAD). The present study examines the cellular defects in vivo and pathologic changes observed in SAD-1 mice at atmospheric oxygenation as well as the effect of acute hypoxia. The transgenic mice showed generalized congestion and microvascular occlusions, occasionally with thrombosis and infarctions of lung, kidneys, penis, and myocardium. The most prevalent chronic organ lesions were congestive splenomegaly (83% of animals) and renal glomerulopathy, which affected 75% of animals by 10 months of age. Further, SAD mice have a mean lifespan that was reduced by 40% when compared with nontransgenic Iittermates. Premature death of SAD mice was associated with acute vasoocclusive events or severe renal disease. SAD mice developed lethal vasoocclusive processes when exposed to reduced pO2 conditions, whereas control mice survived normally. The sensitivity to hypoxia appears to depend on the cellular level of HbSAD, because death occurred at pO2 of 42 mmHg for SAD mice and 49 mmHg for β-thal/SAD. Administration of an antisickling agent that increases oxygen affinity (BW12C79) protected SAD and β-thal/SAD mice from the lethal hypoxic stress. In conclusion, the transgenic SAD and β-thal/SAD mice developed a pathophysiology that strongly resembles human sickle cell disease. Moreover, this animal model allows studies on the effect of antisickling agents.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V84.9.3189.3189