Characterisation of Leishmania donovani promastigotes resistant to hexadecylphosphocholine (miltefosine)
Leishmania donovani promastigote lines resistant to hexadecylphosphocholine (HePC, miltefosine) at 2.5, 5.0, 10.0, 20.0 and 40.0 μM were developed in vitro by continuous step-wise drug pressure. The 40 μM line was 15 times more resistant to HePC than the wild-type clone and showed cross-resistance t...
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Veröffentlicht in: | International journal of antimicrobial agents 2003-10, Vol.22 (4), p.380-387 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Leishmania donovani promastigote lines resistant to hexadecylphosphocholine (HePC, miltefosine) at 2.5, 5.0, 10.0, 20.0 and 40.0 μM were developed in vitro by continuous step-wise drug pressure. The 40 μM line was 15 times more resistant to HePC than the wild-type clone and showed cross-resistance to the ether lipid ET-18-OCH
3 (edelfosine) but not to the standard anti-leishmanial drugs. Resistance was stable up to 12 weeks in drug-free culture medium. No amplification of specific genes, including the multidrug resistance P-glycoprotein gene, could be detected in the resistant parasites. |
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ISSN: | 0924-8579 1872-7913 |
DOI: | 10.1016/S0924-8579(03)00125-0 |