Influence of Deep Hypothermia on the Tolerance of the Isolated Cardiomyocyte to Ischemia–Reperfusion

The influence of deep hypothermia (4°C) during a substrate-free, hypoxia–reoxygenation treatment was investigated on cardiomyocytes (CM) prepared from newborn rat heart in culture in an in vitro, substrate-free model of ischemia–reperfusion. The transmembranous potentials were recorded with standard...

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Veröffentlicht in:Journal of molecular and cellular cardiology 2001-11, Vol.33 (11), p.1973-1988
Hauptverfasser: Bes, Sandrine, Roussel, Pascal, Laubriet, Aline, Vandroux, David, Tissier, Cindy, Rochette, Luc, Athias, Pierre
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container_end_page 1988
container_issue 11
container_start_page 1973
container_title Journal of molecular and cellular cardiology
container_volume 33
creator Bes, Sandrine
Roussel, Pascal
Laubriet, Aline
Vandroux, David
Tissier, Cindy
Rochette, Luc
Athias, Pierre
description The influence of deep hypothermia (4°C) during a substrate-free, hypoxia–reoxygenation treatment was investigated on cardiomyocytes (CM) prepared from newborn rat heart in culture in an in vitro, substrate-free model of ischemia–reperfusion. The transmembranous potentials were recorded with standard microelectrodes. The contractions were monitored photometrically. The RNA messenger (mRNA) and protein expression for protein (HSP70) were analysed by RT-PCR (reverse transcriptase-polymerase chain reaction) and Western blotting, respectively. Simultated ischemia (SI) caused a gradual decrease and then a cessation of the spontaneous electromechanical activity. During the reoxygenation, the CM recovered normal function, provided that SI did not exceed 2.5 h. When SI duration was increased up to 4 h, reoxygenation failed to restore the spontaneous electromechanical activity. Conversely, the exposure of the CM to SI together with deep hypothermia decreased the functional alterations observed, and provided a complete electromechanical recovery after 2.5 h as well as after 4 h of SI. Deep hypothermia alone failed to induce HSP70 mRNA and protein production. On the contrary, HSP70 mRNA production increased after 2.5 and 4 h of deep hypothermia followed by 1 h of rewarming, proportionally to the duration of the cooling period. This augmentation in mRNA was associated with a rise in HSP70 protein content. In summary, it appeared that deep hypothermia exerts a strong cytoprotectrive action during SI only, whereas cooling CM before SI has no beneficial effect on subsequent SI. Moreover, these results suggested the persistence of a signaling system and/or transduction in deeply cooled, functionally depressed cells. Finally, CM in culture appeared to be a model of interest for studying heart graft protection against ischemia-reperfusion and contributed to clarifying the molecular and cellular mechanisms of deep hypothermia on myocardium.
doi_str_mv 10.1006/jmcc.2001.1461
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The transmembranous potentials were recorded with standard microelectrodes. The contractions were monitored photometrically. The RNA messenger (mRNA) and protein expression for protein (HSP70) were analysed by RT-PCR (reverse transcriptase-polymerase chain reaction) and Western blotting, respectively. Simultated ischemia (SI) caused a gradual decrease and then a cessation of the spontaneous electromechanical activity. During the reoxygenation, the CM recovered normal function, provided that SI did not exceed 2.5 h. When SI duration was increased up to 4 h, reoxygenation failed to restore the spontaneous electromechanical activity. Conversely, the exposure of the CM to SI together with deep hypothermia decreased the functional alterations observed, and provided a complete electromechanical recovery after 2.5 h as well as after 4 h of SI. Deep hypothermia alone failed to induce HSP70 mRNA and protein production. On the contrary, HSP70 mRNA production increased after 2.5 and 4 h of deep hypothermia followed by 1 h of rewarming, proportionally to the duration of the cooling period. This augmentation in mRNA was associated with a rise in HSP70 protein content. In summary, it appeared that deep hypothermia exerts a strong cytoprotectrive action during SI only, whereas cooling CM before SI has no beneficial effect on subsequent SI. Moreover, these results suggested the persistence of a signaling system and/or transduction in deeply cooled, functionally depressed cells. 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On the contrary, HSP70 mRNA production increased after 2.5 and 4 h of deep hypothermia followed by 1 h of rewarming, proportionally to the duration of the cooling period. This augmentation in mRNA was associated with a rise in HSP70 protein content. In summary, it appeared that deep hypothermia exerts a strong cytoprotectrive action during SI only, whereas cooling CM before SI has no beneficial effect on subsequent SI. Moreover, these results suggested the persistence of a signaling system and/or transduction in deeply cooled, functionally depressed cells. 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Roussel, Pascal ; Laubriet, Aline ; Vandroux, David ; Tissier, Cindy ; Rochette, Luc ; Athias, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-c663ec4228fe57e8b63a03e41a8e4a9f3b7c626311e399ce58924cc51bea152f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cardiomyocytes</topic><topic>Cells, Cultured</topic><topic>Cellular contractions</topic><topic>Cellular culture</topic><topic>Deep hypothermia</topic><topic>Electrophysiology</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>HSP70</topic><topic>HSP70 Heat-Shock Proteins - metabolism</topic><topic>Hypothermia</topic><topic>Ischemia - metabolism</topic><topic>Messenger RNA</topic><topic>Myocardial Ischemia - prevention &amp; control</topic><topic>Myocardial Reperfusion Injury - prevention &amp; control</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Simulated ischemia–reperfusion</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bes, Sandrine</creatorcontrib><creatorcontrib>Roussel, Pascal</creatorcontrib><creatorcontrib>Laubriet, Aline</creatorcontrib><creatorcontrib>Vandroux, David</creatorcontrib><creatorcontrib>Tissier, Cindy</creatorcontrib><creatorcontrib>Rochette, Luc</creatorcontrib><creatorcontrib>Athias, Pierre</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular and cellular cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bes, Sandrine</au><au>Roussel, Pascal</au><au>Laubriet, Aline</au><au>Vandroux, David</au><au>Tissier, Cindy</au><au>Rochette, Luc</au><au>Athias, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of Deep Hypothermia on the Tolerance of the Isolated Cardiomyocyte to Ischemia–Reperfusion</atitle><jtitle>Journal of molecular and cellular cardiology</jtitle><addtitle>J Mol Cell Cardiol</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>33</volume><issue>11</issue><spage>1973</spage><epage>1988</epage><pages>1973-1988</pages><issn>0022-2828</issn><eissn>1095-8584</eissn><abstract>The influence of deep hypothermia (4°C) during a substrate-free, hypoxia–reoxygenation treatment was investigated on cardiomyocytes (CM) prepared from newborn rat heart in culture in an in vitro, substrate-free model of ischemia–reperfusion. The transmembranous potentials were recorded with standard microelectrodes. The contractions were monitored photometrically. The RNA messenger (mRNA) and protein expression for protein (HSP70) were analysed by RT-PCR (reverse transcriptase-polymerase chain reaction) and Western blotting, respectively. Simultated ischemia (SI) caused a gradual decrease and then a cessation of the spontaneous electromechanical activity. During the reoxygenation, the CM recovered normal function, provided that SI did not exceed 2.5 h. When SI duration was increased up to 4 h, reoxygenation failed to restore the spontaneous electromechanical activity. Conversely, the exposure of the CM to SI together with deep hypothermia decreased the functional alterations observed, and provided a complete electromechanical recovery after 2.5 h as well as after 4 h of SI. Deep hypothermia alone failed to induce HSP70 mRNA and protein production. On the contrary, HSP70 mRNA production increased after 2.5 and 4 h of deep hypothermia followed by 1 h of rewarming, proportionally to the duration of the cooling period. This augmentation in mRNA was associated with a rise in HSP70 protein content. In summary, it appeared that deep hypothermia exerts a strong cytoprotectrive action during SI only, whereas cooling CM before SI has no beneficial effect on subsequent SI. Moreover, these results suggested the persistence of a signaling system and/or transduction in deeply cooled, functionally depressed cells. Finally, CM in culture appeared to be a model of interest for studying heart graft protection against ischemia-reperfusion and contributed to clarifying the molecular and cellular mechanisms of deep hypothermia on myocardium.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11708842</pmid><doi>10.1006/jmcc.2001.1461</doi><tpages>16</tpages></addata></record>
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subjects Animals
Blotting, Western
Cardiomyocytes
Cells, Cultured
Cellular contractions
Cellular culture
Deep hypothermia
Electrophysiology
Heat-Shock Proteins - metabolism
HSP70
HSP70 Heat-Shock Proteins - metabolism
Hypothermia
Ischemia - metabolism
Messenger RNA
Myocardial Ischemia - prevention & control
Myocardial Reperfusion Injury - prevention & control
Myocardium - cytology
Myocardium - metabolism
Rat
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
RNA - metabolism
RNA, Messenger - metabolism
Simulated ischemia–reperfusion
Temperature
Time Factors
title Influence of Deep Hypothermia on the Tolerance of the Isolated Cardiomyocyte to Ischemia–Reperfusion
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