Pendrin Is an Iodide-Specific Apical Porter Responsible for Iodide Efflux from Thyroid Cells

The Pendred syndrome gene encodes a 780-amino acid putative transmembrane protein (pendrin) that is expressed in the apical membrane of thyroid follicular cells. Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using ma...

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Veröffentlicht in:The journal of clinical endocrinology and metabolism 2002-07, Vol.87 (7), p.3356-3361
Hauptverfasser: Yoshida, Akio, Taniguchi, Shinichi, Hisatome, Ichiro, Royaux, Ines E., Green, Eric D., Kohn, Leonard D., Suzuki, Koichi
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Sprache:eng
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Zusammenfassung:The Pendred syndrome gene encodes a 780-amino acid putative transmembrane protein (pendrin) that is expressed in the apical membrane of thyroid follicular cells. Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using mammalian cells to study its role in thyroid function. We show here, using COS-7 cells and Chinese hamster ovary cells transfected with expression vectors encoding sodium iodide symporter or human Pendred syndrome gene cDNA and by comparison with studies using rat thyroid FRTL-5 cells, that pendrin is an iodide-specific transporter in mammalian cells and is responsible for iodide efflux in the thyroid.
ISSN:0021-972X
1945-7197
DOI:10.1210/jcem.87.7.8679