Pendrin Is an Iodide-Specific Apical Porter Responsible for Iodide Efflux from Thyroid Cells
The Pendred syndrome gene encodes a 780-amino acid putative transmembrane protein (pendrin) that is expressed in the apical membrane of thyroid follicular cells. Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using ma...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2002-07, Vol.87 (7), p.3356-3361 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | Yoshida, Akio Taniguchi, Shinichi Hisatome, Ichiro Royaux, Ines E. Green, Eric D. Kohn, Leonard D. Suzuki, Koichi |
| description | The Pendred syndrome gene encodes a 780-amino acid putative transmembrane protein (pendrin) that is expressed in the apical membrane of thyroid follicular cells. Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using mammalian cells to study its role in thyroid function. We show here, using COS-7 cells and Chinese hamster ovary cells transfected with expression vectors encoding sodium iodide symporter or human Pendred syndrome gene cDNA and by comparison with studies using rat thyroid FRTL-5 cells, that pendrin is an iodide-specific transporter in mammalian cells and is responsible for iodide efflux in the thyroid. |
| doi_str_mv | 10.1210/jcem.87.7.8679 |
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Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using mammalian cells to study its role in thyroid function. We show here, using COS-7 cells and Chinese hamster ovary cells transfected with expression vectors encoding sodium iodide symporter or human Pendred syndrome gene cDNA and by comparison with studies using rat thyroid FRTL-5 cells, that pendrin is an iodide-specific transporter in mammalian cells and is responsible for iodide efflux in the thyroid.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jcem.87.7.8679</identifier><identifier>PMID: 12107249</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Binding and carrier proteins ; Biological and medical sciences ; Biological Transport - physiology ; Carrier Proteins - physiology ; Cell Line ; Cell Membrane - metabolism ; Chlorides - pharmacology ; CHO Cells ; COS Cells ; Cricetinae ; Endocrinopathies ; Fundamental and applied biological sciences. Psychology ; Humans ; Iodides - pharmacokinetics ; Iodine - pharmacokinetics ; Iodine Radioisotopes ; Medical sciences ; Membrane Transport Proteins ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Proteins ; Rats ; Symporters - physiology ; Thyroid Gland - cytology ; Thyroid Gland - metabolism ; Thyroid. 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Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using mammalian cells to study its role in thyroid function. We show here, using COS-7 cells and Chinese hamster ovary cells transfected with expression vectors encoding sodium iodide symporter or human Pendred syndrome gene cDNA and by comparison with studies using rat thyroid FRTL-5 cells, that pendrin is an iodide-specific transporter in mammalian cells and is responsible for iodide efflux in the thyroid.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - physiology</subject><subject>Carrier Proteins - physiology</subject><subject>Cell Line</subject><subject>Cell Membrane - metabolism</subject><subject>Chlorides - pharmacology</subject><subject>CHO Cells</subject><subject>COS Cells</subject><subject>Cricetinae</subject><subject>Endocrinopathies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Iodides - pharmacokinetics</subject><subject>Iodine - pharmacokinetics</subject><subject>Iodine Radioisotopes</subject><subject>Medical sciences</subject><subject>Membrane Transport Proteins</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Proteins</subject><subject>Rats</subject><subject>Symporters - physiology</subject><subject>Thyroid Gland - cytology</subject><subject>Thyroid Gland - metabolism</subject><subject>Thyroid. Thyroid axis (diseases)</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10DFr3TAQwHERWpLXtGvGoKXd7Eq25LPG8HhtA4GGNoUOBSHLJ6KHbTlSDM23r8wzZOoiLb87jj8hV5yVvOLs89HiWLZQQtk2oM7IjishC-AK3pAdYxUvFFS_L8i7lI6McSFkfU4u1lGohNqRP_c49dFP9DZRk9_Q-x6LnzNa77ylN7O3ZqD3IT5jpD8wzWFKvhuQuhA3TQ_ODctf6mIY6cPjSwy-p3schvSevHVmSPhh-y_Jry-Hh_234u7719v9zV1h67ZVBXSsYc5AVVuGUoKEvmaiRgvKcYm2M03tjOpU18gWee8Ys64CJSrjeCeb-pJ8Ou2dY3haMD3r0SebLzAThiVp4K2SQqywPEEbQ0oRnZ6jH0180ZzpNYpee-oWNOi1Zx643jYv3Yj9K98CZvBxAyblUi6ayfr06mqAVoDITp5czh1sDo5zxJT0MSxxym3-d8A_u-mQXw</recordid><startdate>200207</startdate><enddate>200207</enddate><creator>Yoshida, Akio</creator><creator>Taniguchi, Shinichi</creator><creator>Hisatome, Ichiro</creator><creator>Royaux, Ines E.</creator><creator>Green, Eric D.</creator><creator>Kohn, Leonard D.</creator><creator>Suzuki, Koichi</creator><general>Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200207</creationdate><title>Pendrin Is an Iodide-Specific Apical Porter Responsible for Iodide Efflux from Thyroid Cells</title><author>Yoshida, Akio ; Taniguchi, Shinichi ; Hisatome, Ichiro ; Royaux, Ines E. ; Green, Eric D. ; Kohn, Leonard D. ; Suzuki, Koichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3889-7b060fa723c0e55757d3043ec79f15ecba63fa9b9b658e1df00cf27942af1b563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Binding and carrier proteins</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - physiology</topic><topic>Carrier Proteins - physiology</topic><topic>Cell Line</topic><topic>Cell Membrane - metabolism</topic><topic>Chlorides - pharmacology</topic><topic>CHO Cells</topic><topic>COS Cells</topic><topic>Cricetinae</topic><topic>Endocrinopathies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Iodides - pharmacokinetics</topic><topic>Iodine - pharmacokinetics</topic><topic>Iodine Radioisotopes</topic><topic>Medical sciences</topic><topic>Membrane Transport Proteins</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Proteins</topic><topic>Rats</topic><topic>Symporters - physiology</topic><topic>Thyroid Gland - cytology</topic><topic>Thyroid Gland - metabolism</topic><topic>Thyroid. Thyroid axis (diseases)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshida, Akio</creatorcontrib><creatorcontrib>Taniguchi, Shinichi</creatorcontrib><creatorcontrib>Hisatome, Ichiro</creatorcontrib><creatorcontrib>Royaux, Ines E.</creatorcontrib><creatorcontrib>Green, Eric D.</creatorcontrib><creatorcontrib>Kohn, Leonard D.</creatorcontrib><creatorcontrib>Suzuki, Koichi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshida, Akio</au><au>Taniguchi, Shinichi</au><au>Hisatome, Ichiro</au><au>Royaux, Ines E.</au><au>Green, Eric D.</au><au>Kohn, Leonard D.</au><au>Suzuki, Koichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pendrin Is an Iodide-Specific Apical Porter Responsible for Iodide Efflux from Thyroid Cells</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2002-07</date><risdate>2002</risdate><volume>87</volume><issue>7</issue><spage>3356</spage><epage>3361</epage><pages>3356-3361</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>The Pendred syndrome gene encodes a 780-amino acid putative transmembrane protein (pendrin) that is expressed in the apical membrane of thyroid follicular cells. Although pendrin was shown to transport iodide and chloride using Xenopus laevis oocytes and Sf9 insect cells, there is no report using mammalian cells to study its role in thyroid function. We show here, using COS-7 cells and Chinese hamster ovary cells transfected with expression vectors encoding sodium iodide symporter or human Pendred syndrome gene cDNA and by comparison with studies using rat thyroid FRTL-5 cells, that pendrin is an iodide-specific transporter in mammalian cells and is responsible for iodide efflux in the thyroid.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>12107249</pmid><doi>10.1210/jcem.87.7.8679</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Analytical, structural and metabolic biochemistry Animals Binding and carrier proteins Biological and medical sciences Biological Transport - physiology Carrier Proteins - physiology Cell Line Cell Membrane - metabolism Chlorides - pharmacology CHO Cells COS Cells Cricetinae Endocrinopathies Fundamental and applied biological sciences. Psychology Humans Iodides - pharmacokinetics Iodine - pharmacokinetics Iodine Radioisotopes Medical sciences Membrane Transport Proteins Non tumoral diseases. Target tissue resistance. Benign neoplasms Proteins Rats Symporters - physiology Thyroid Gland - cytology Thyroid Gland - metabolism Thyroid. Thyroid axis (diseases) |
| title | Pendrin Is an Iodide-Specific Apical Porter Responsible for Iodide Efflux from Thyroid Cells |
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