Phenotypic analysis of a novel chordin mutant in medaka

We have isolated and characterized a ventralized mutant in medaka (the Japanese killifish; Oryzias latipes), which turned out to have a mutation in the chordin gene. The mutant exhibits ventralization of the body axis, malformation of axial bones, over‐bifurcation of yolk sac blood vessels, and late...

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Veröffentlicht in:Developmental dynamics 2007-08, Vol.236 (8), p.2298-2310
Hauptverfasser: Takashima, Shigeo, Shimada, Atsuko, Kobayashi, Daisuke, Yokoi, Hayato, Narita, Takanori, Jindo, Tomoko, Kage, Takahiro, Kitagawa, Tadao, Kimura, Tetsuaki, Sekimizu, Koshin, Miyake, Akimitsu, Setiamarga, Davin H.E., Murakami, Ryohei, Tsuda, Sachiko, Ooki, Shinya, Kakihara, Ken, Hojo, Motoki, Naruse, Kiyoshi, Mitani, Hiroshi, Shima, Akihiro, Ishikawa, Yuji, Araki, Kazuo, Saga, Yumiko, Takeda, Hiroyuki
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Sprache:eng
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Zusammenfassung:We have isolated and characterized a ventralized mutant in medaka (the Japanese killifish; Oryzias latipes), which turned out to have a mutation in the chordin gene. The mutant exhibits ventralization of the body axis, malformation of axial bones, over‐bifurcation of yolk sac blood vessels, and laterality defects in internal organs. The mutant exhibits variability of phenotypes, depending on the culture temperature, from embryos with a slightly ventralized phenotype to those without any head and trunk structures. Taking advantages of these variable and severe phenotypes, we analyzed the role of Chordin‐dependent tissues such as the notochord and Kupffer's vesicle (KV) in the establishment of left–right axis in fish. The results demonstrate that, in the absence of the notochord and KV, the medaka lateral plate mesoderm autonomously and bilaterally expresses spaw gene in a default state. Developmental Dynamics 236:2298–2310, 2007. © 2007 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.21245