Influence of InVitro Digestion on Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Activity of Plant-Protein Hydrolysates Obtained from Agro-Industrial By-Products

This study investigates the production of protein hydrolysates with dipeptidyl peptidase-IV (DPP-IV) inhibitory activity from agro-industrial by-products, namely olive seed, sunflower seed, rapeseed, and lupin meals, as well as from two plant protein isolates such as pea and potato. Furthermore, the...

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Hauptverfasser: Pérez-Gálvez, Raúl, Berraquero-García, Carmen, Ospina-Quiroga, J Lizeth, Espejo-Carpio, F Javier, Almécija, M Carmen, Guadix, Antonio, García-Moreno, Pedro J, Guadix, Emilia M
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container_start_page
container_title Foods
container_volume 13
creator Pérez-Gálvez, Raúl
Berraquero-García, Carmen
Ospina-Quiroga, J Lizeth
Espejo-Carpio, F Javier
Almécija, M Carmen
Guadix, Antonio
García-Moreno, Pedro J
Guadix, Emilia M
description This study investigates the production of protein hydrolysates with dipeptidyl peptidase-IV (DPP-IV) inhibitory activity from agro-industrial by-products, namely olive seed, sunflower seed, rapeseed, and lupin meals, as well as from two plant protein isolates such as pea and potato. Furthermore, the effect of simulated gastrointestinal digestion on the DPP-IV inhibitory activity of all the hydrolysates was evaluated. Overall, the lowest values of IC50 (1.02 ± 0.09 - 1.24 ± 0.19 mg protein/mL) were observed for the hydrolysates with a high proportion of short-chain [< 1 kDa] peptides (i.e., olive seed, sunflower seed, and lupin) or high content of proline (i.e., rapeseed). Contrarily, the IC50 of the pea and potato hydrolysates was significantly higher (1.50 ± 0.13 - 1.93 ± 0.13 mg protein/mL). In vitro digestion led to an increase in peptides
doi_str_mv 10.3390/foods13172691
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Furthermore, the effect of simulated gastrointestinal digestion on the DPP-IV inhibitory activity of all the hydrolysates was evaluated. Overall, the lowest values of IC50 (1.02 ± 0.09 - 1.24 ± 0.19 mg protein/mL) were observed for the hydrolysates with a high proportion of short-chain [&lt; 1 kDa] peptides (i.e., olive seed, sunflower seed, and lupin) or high content of proline (i.e., rapeseed). Contrarily, the IC50 of the pea and potato hydrolysates was significantly higher (1.50 ± 0.13 - 1.93 ± 0.13 mg protein/mL). In vitro digestion led to an increase in peptides &lt;1 kDa for almost all hydrolysates (except olive and sunflower seed meals), which was noticeable for rapeseed, pea, and potato hydrolysates. Digestion did not significantly modify the DPP-IV inhibitory activity of olive, sunflower, rapeseed, and potato hydrolysates, whereas a significant decrease in IC50 value was obtained for pea hydrolysate and a significant increase in IC50 was obtained for lupin hydrolysate. Thus, this work shows the potential of agro-industrial by-products for the production of protein hydrolysates exhibiting DPP-IV inhibition.This study investigates the production of protein hydrolysates with dipeptidyl peptidase-IV (DPP-IV) inhibitory activity from agro-industrial by-products, namely olive seed, sunflower seed, rapeseed, and lupin meals, as well as from two plant protein isolates such as pea and potato. Furthermore, the effect of simulated gastrointestinal digestion on the DPP-IV inhibitory activity of all the hydrolysates was evaluated. Overall, the lowest values of IC50 (1.02 ± 0.09 - 1.24 ± 0.19 mg protein/mL) were observed for the hydrolysates with a high proportion of short-chain [&lt; 1 kDa] peptides (i.e., olive seed, sunflower seed, and lupin) or high content of proline (i.e., rapeseed). Contrarily, the IC50 of the pea and potato hydrolysates was significantly higher (1.50 ± 0.13 - 1.93 ± 0.13 mg protein/mL). In vitro digestion led to an increase in peptides &lt;1 kDa for almost all hydrolysates (except olive and sunflower seed meals), which was noticeable for rapeseed, pea, and potato hydrolysates. Digestion did not significantly modify the DPP-IV inhibitory activity of olive, sunflower, rapeseed, and potato hydrolysates, whereas a significant decrease in IC50 value was obtained for pea hydrolysate and a significant increase in IC50 was obtained for lupin hydrolysate. Thus, this work shows the potential of agro-industrial by-products for the production of protein hydrolysates exhibiting DPP-IV inhibition.</description><identifier>ISSN: 2304-8158</identifier><identifier>EISSN: 2304-8158</identifier><identifier>DOI: 10.3390/foods13172691</identifier><language>eng</language><subject>digestion ; dipeptidyl-peptidase IV ; Helianthus annuus ; hydrolysates ; in vitro digestion ; Lupinus ; olives ; peas ; peptides ; plant proteins ; potatoes ; proline ; rapeseed ; sunflower seed</subject><ispartof>Foods, 2024-08, Vol.13 (17)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>Pérez-Gálvez, Raúl</creatorcontrib><creatorcontrib>Berraquero-García, Carmen</creatorcontrib><creatorcontrib>Ospina-Quiroga, J Lizeth</creatorcontrib><creatorcontrib>Espejo-Carpio, F Javier</creatorcontrib><creatorcontrib>Almécija, M Carmen</creatorcontrib><creatorcontrib>Guadix, Antonio</creatorcontrib><creatorcontrib>García-Moreno, Pedro J</creatorcontrib><creatorcontrib>Guadix, Emilia M</creatorcontrib><title>Influence of InVitro Digestion on Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Activity of Plant-Protein Hydrolysates Obtained from Agro-Industrial By-Products</title><title>Foods</title><description>This study investigates the production of protein hydrolysates with dipeptidyl peptidase-IV (DPP-IV) inhibitory activity from agro-industrial by-products, namely olive seed, sunflower seed, rapeseed, and lupin meals, as well as from two plant protein isolates such as pea and potato. Furthermore, the effect of simulated gastrointestinal digestion on the DPP-IV inhibitory activity of all the hydrolysates was evaluated. Overall, the lowest values of IC50 (1.02 ± 0.09 - 1.24 ± 0.19 mg protein/mL) were observed for the hydrolysates with a high proportion of short-chain [&lt; 1 kDa] peptides (i.e., olive seed, sunflower seed, and lupin) or high content of proline (i.e., rapeseed). Contrarily, the IC50 of the pea and potato hydrolysates was significantly higher (1.50 ± 0.13 - 1.93 ± 0.13 mg protein/mL). In vitro digestion led to an increase in peptides &lt;1 kDa for almost all hydrolysates (except olive and sunflower seed meals), which was noticeable for rapeseed, pea, and potato hydrolysates. Digestion did not significantly modify the DPP-IV inhibitory activity of olive, sunflower, rapeseed, and potato hydrolysates, whereas a significant decrease in IC50 value was obtained for pea hydrolysate and a significant increase in IC50 was obtained for lupin hydrolysate. Thus, this work shows the potential of agro-industrial by-products for the production of protein hydrolysates exhibiting DPP-IV inhibition.This study investigates the production of protein hydrolysates with dipeptidyl peptidase-IV (DPP-IV) inhibitory activity from agro-industrial by-products, namely olive seed, sunflower seed, rapeseed, and lupin meals, as well as from two plant protein isolates such as pea and potato. Furthermore, the effect of simulated gastrointestinal digestion on the DPP-IV inhibitory activity of all the hydrolysates was evaluated. Overall, the lowest values of IC50 (1.02 ± 0.09 - 1.24 ± 0.19 mg protein/mL) were observed for the hydrolysates with a high proportion of short-chain [&lt; 1 kDa] peptides (i.e., olive seed, sunflower seed, and lupin) or high content of proline (i.e., rapeseed). Contrarily, the IC50 of the pea and potato hydrolysates was significantly higher (1.50 ± 0.13 - 1.93 ± 0.13 mg protein/mL). In vitro digestion led to an increase in peptides &lt;1 kDa for almost all hydrolysates (except olive and sunflower seed meals), which was noticeable for rapeseed, pea, and potato hydrolysates. Digestion did not significantly modify the DPP-IV inhibitory activity of olive, sunflower, rapeseed, and potato hydrolysates, whereas a significant decrease in IC50 value was obtained for pea hydrolysate and a significant increase in IC50 was obtained for lupin hydrolysate. Thus, this work shows the potential of agro-industrial by-products for the production of protein hydrolysates exhibiting DPP-IV inhibition.</description><subject>digestion</subject><subject>dipeptidyl-peptidase IV</subject><subject>Helianthus annuus</subject><subject>hydrolysates</subject><subject>in vitro digestion</subject><subject>Lupinus</subject><subject>olives</subject><subject>peas</subject><subject>peptides</subject><subject>plant proteins</subject><subject>potatoes</subject><subject>proline</subject><subject>rapeseed</subject><subject>sunflower seed</subject><issn>2304-8158</issn><issn>2304-8158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNULFOwzAUtBBIVKUju8cyBOy4jp2xtEAjVWqGqmvlxC_FKLVL7CDlZ_hWXGBg5OlJd8PdSXcI3VJyz1hOHhrntKeMijTL6QUapYzMEkm5vPzDr9HE-zcSL6dMsnSEPgvbtD3YGrBrcGF3JnQOL80BfDDO4vhLc4JTMHpocflNlIek2OHpsiwj3kXXq6lMcN2A53UwHyYM57CyVTYkZecCGItXg-5cO3gVwONNFZSxoHHTuSOeHzqXFFb3PnRGtfhxOLt0Xwd_g64a1XqY_OIYbZ-ftotVst68FIv5OjllGUtykkqeVkRQXeUcsopoKhqoQVVasTiP5BSIkFxIpYSEFLJGK8WlrmQjo2KMpj-xp86997H6_mh8DW1sAK73e0Y5E1k-Y_-RkhlnUuSEfQEf-X32</recordid><startdate>20240826</startdate><enddate>20240826</enddate><creator>Pérez-Gálvez, Raúl</creator><creator>Berraquero-García, Carmen</creator><creator>Ospina-Quiroga, J Lizeth</creator><creator>Espejo-Carpio, F Javier</creator><creator>Almécija, M Carmen</creator><creator>Guadix, Antonio</creator><creator>García-Moreno, Pedro J</creator><creator>Guadix, Emilia M</creator><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240826</creationdate><title>Influence of InVitro Digestion on Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Activity of Plant-Protein Hydrolysates Obtained from Agro-Industrial By-Products</title><author>Pérez-Gálvez, Raúl ; Berraquero-García, Carmen ; Ospina-Quiroga, J Lizeth ; Espejo-Carpio, F Javier ; Almécija, M Carmen ; Guadix, Antonio ; García-Moreno, Pedro J ; Guadix, Emilia M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p663-902852b071db95e6b0d17feceabda3339851e078578aa78e2e6fdaa58db8f8a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>digestion</topic><topic>dipeptidyl-peptidase IV</topic><topic>Helianthus annuus</topic><topic>hydrolysates</topic><topic>in vitro digestion</topic><topic>Lupinus</topic><topic>olives</topic><topic>peas</topic><topic>peptides</topic><topic>plant proteins</topic><topic>potatoes</topic><topic>proline</topic><topic>rapeseed</topic><topic>sunflower seed</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pérez-Gálvez, Raúl</creatorcontrib><creatorcontrib>Berraquero-García, Carmen</creatorcontrib><creatorcontrib>Ospina-Quiroga, J Lizeth</creatorcontrib><creatorcontrib>Espejo-Carpio, F Javier</creatorcontrib><creatorcontrib>Almécija, M Carmen</creatorcontrib><creatorcontrib>Guadix, Antonio</creatorcontrib><creatorcontrib>García-Moreno, Pedro J</creatorcontrib><creatorcontrib>Guadix, Emilia M</creatorcontrib><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Foods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez-Gálvez, Raúl</au><au>Berraquero-García, Carmen</au><au>Ospina-Quiroga, J Lizeth</au><au>Espejo-Carpio, F Javier</au><au>Almécija, M Carmen</au><au>Guadix, Antonio</au><au>García-Moreno, Pedro J</au><au>Guadix, Emilia M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of InVitro Digestion on Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Activity of Plant-Protein Hydrolysates Obtained from Agro-Industrial By-Products</atitle><jtitle>Foods</jtitle><date>2024-08-26</date><risdate>2024</risdate><volume>13</volume><issue>17</issue><issn>2304-8158</issn><eissn>2304-8158</eissn><abstract>This study investigates the production of protein hydrolysates with dipeptidyl peptidase-IV (DPP-IV) inhibitory activity from agro-industrial by-products, namely olive seed, sunflower seed, rapeseed, and lupin meals, as well as from two plant protein isolates such as pea and potato. Furthermore, the effect of simulated gastrointestinal digestion on the DPP-IV inhibitory activity of all the hydrolysates was evaluated. Overall, the lowest values of IC50 (1.02 ± 0.09 - 1.24 ± 0.19 mg protein/mL) were observed for the hydrolysates with a high proportion of short-chain [&lt; 1 kDa] peptides (i.e., olive seed, sunflower seed, and lupin) or high content of proline (i.e., rapeseed). Contrarily, the IC50 of the pea and potato hydrolysates was significantly higher (1.50 ± 0.13 - 1.93 ± 0.13 mg protein/mL). In vitro digestion led to an increase in peptides &lt;1 kDa for almost all hydrolysates (except olive and sunflower seed meals), which was noticeable for rapeseed, pea, and potato hydrolysates. Digestion did not significantly modify the DPP-IV inhibitory activity of olive, sunflower, rapeseed, and potato hydrolysates, whereas a significant decrease in IC50 value was obtained for pea hydrolysate and a significant increase in IC50 was obtained for lupin hydrolysate. Thus, this work shows the potential of agro-industrial by-products for the production of protein hydrolysates exhibiting DPP-IV inhibition.This study investigates the production of protein hydrolysates with dipeptidyl peptidase-IV (DPP-IV) inhibitory activity from agro-industrial by-products, namely olive seed, sunflower seed, rapeseed, and lupin meals, as well as from two plant protein isolates such as pea and potato. Furthermore, the effect of simulated gastrointestinal digestion on the DPP-IV inhibitory activity of all the hydrolysates was evaluated. Overall, the lowest values of IC50 (1.02 ± 0.09 - 1.24 ± 0.19 mg protein/mL) were observed for the hydrolysates with a high proportion of short-chain [&lt; 1 kDa] peptides (i.e., olive seed, sunflower seed, and lupin) or high content of proline (i.e., rapeseed). Contrarily, the IC50 of the pea and potato hydrolysates was significantly higher (1.50 ± 0.13 - 1.93 ± 0.13 mg protein/mL). In vitro digestion led to an increase in peptides &lt;1 kDa for almost all hydrolysates (except olive and sunflower seed meals), which was noticeable for rapeseed, pea, and potato hydrolysates. Digestion did not significantly modify the DPP-IV inhibitory activity of olive, sunflower, rapeseed, and potato hydrolysates, whereas a significant decrease in IC50 value was obtained for pea hydrolysate and a significant increase in IC50 was obtained for lupin hydrolysate. Thus, this work shows the potential of agro-industrial by-products for the production of protein hydrolysates exhibiting DPP-IV inhibition.</abstract><doi>10.3390/foods13172691</doi></addata></record>
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source MDPI - Multidisciplinary Digital Publishing Institute; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects digestion
dipeptidyl-peptidase IV
Helianthus annuus
hydrolysates
in vitro digestion
Lupinus
olives
peas
peptides
plant proteins
potatoes
proline
rapeseed
sunflower seed
title Influence of InVitro Digestion on Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Activity of Plant-Protein Hydrolysates Obtained from Agro-Industrial By-Products
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