High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina
Background Ovine anaplasmosis ( sensu stricto ) is a rickettsial blood disease caused by the tick-borne species Anaplasma ovis . The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the pre...
Gespeichert in:
Veröffentlicht in: | Molecular biology reports 2024-12, Vol.51 (1), p.936-936, Article 936 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Background
Ovine anaplasmosis (
sensu stricto
) is a rickettsial blood disease caused by the tick-borne species
Anaplasma ovis
. The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the presence of
Anaplasma ovis
in Bosnia and Herzegovina. However, given the country’s location within the Mediterranean Basin and the recent molecular detection of
Babesia ovis
, it is plausible that sheep in the region could naturally be infected with this tick-borne pathogen.
Methods and results
Blood samples from 81 sheep in the Podrinje and Herzegovina areas were examined by PCR. PCR positivity was found in 38 (46.9%) cases indicating a high number of infected sheep. Mixed infections with
Babesia ovis
and
A.ovis
were observed in 63.3% of cases. A higher number of positive sheep was recorded in the area of Herzegovina. Phylogenetic analysis of the
gltA
,
groEL
, and
msp4
genes of
A. ovis
revealed numerous genotypes and significant genetic variability. This diversity was not related to geographic origin, tick-borne infection status, or sheep breeding practices in Podrinje and Herzegovina.
Conclusions
The data obtained in this study suggest that the emergence of new genotypes and the high genetic variability of
A. ovis
are driven by specific local and micro-environmental factors. |
---|---|
ISSN: | 0301-4851 1573-4978 1573-4978 |
DOI: | 10.1007/s11033-024-09869-9 |