High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina
Background Ovine anaplasmosis ( sensu stricto ) is a rickettsial blood disease caused by the tick-borne species Anaplasma ovis . The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the pre...
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| creator | Stevanović, Oliver Ilić, Tamara Jovanović, Nemanja Vejnović, Branislav Radalj, Andrea |
| description | Background
Ovine anaplasmosis (
sensu stricto
) is a rickettsial blood disease caused by the tick-borne species
Anaplasma ovis
. The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the presence of
Anaplasma ovis
in Bosnia and Herzegovina. However, given the country’s location within the Mediterranean Basin and the recent molecular detection of
Babesia ovis
, it is plausible that sheep in the region could naturally be infected with this tick-borne pathogen.
Methods and results
Blood samples from 81 sheep in the Podrinje and Herzegovina areas were examined by PCR. PCR positivity was found in 38 (46.9%) cases indicating a high number of infected sheep. Mixed infections with
Babesia ovis
and
A.ovis
were observed in 63.3% of cases. A higher number of positive sheep was recorded in the area of Herzegovina. Phylogenetic analysis of the
gltA
,
groEL
, and
msp4
genes of
A. ovis
revealed numerous genotypes and significant genetic variability. This diversity was not related to geographic origin, tick-borne infection status, or sheep breeding practices in Podrinje and Herzegovina.
Conclusions
The data obtained in this study suggest that the emergence of new genotypes and the high genetic variability of
A. ovis
are driven by specific local and micro-environmental factors. |
| doi_str_mv | 10.1007/s11033-024-09869-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153702726</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153702726</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-7c5812baff66868719c96b359510e3b96993e30d7c074bc757d3413e6d9592b43</originalsourceid><addsrcrecordid>eNqFkT1vFDEQhi0EIkfCH0gRWaKhWZjx95RJBBxSEA2pLe-u9-Lo1nux7yKFX88eF4iUAqop5nnf0ehh7BThAwLYjxURpGxAqAbIGWroBVugtrJRZN1LtgAJ2Cin8Yi9qfUWABRa_ZodSUInBOCCfVum1Q1fxRy3qeN9uo-lpu0DnwZ-nsNmHeoY-HSfKk-Z15sYN3wo08gvpppT4CH3fBnLz7iamRxO2KshrGt8-ziP2fXnTz8ul83V9y9fL8-vmk5q2ja20w5FG4bBGGecRerItPNKI0TZkiGSUUJvO7Cq7ay2vVQoo-lJk2iVPGbvD72bMt3tYt36MdUurtchx2lXvUQtLQgrzP9RIIuKhHIz-u4ZejvtSp4f2VPGIjraF4oD1ZWp1hIHvylpDOXBI_i9F3_w4mcv_rcXT3Po7LF6146x_xv5I2IG5AGo8yqvYnm6_Y_aXz9OlYA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3096711896</pqid></control><display><type>article</type><title>High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Stevanović, Oliver ; Ilić, Tamara ; Jovanović, Nemanja ; Vejnović, Branislav ; Radalj, Andrea</creator><creatorcontrib>Stevanović, Oliver ; Ilić, Tamara ; Jovanović, Nemanja ; Vejnović, Branislav ; Radalj, Andrea</creatorcontrib><description>Background
Ovine anaplasmosis (
sensu stricto
) is a rickettsial blood disease caused by the tick-borne species
Anaplasma ovis
. The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the presence of
Anaplasma ovis
in Bosnia and Herzegovina. However, given the country’s location within the Mediterranean Basin and the recent molecular detection of
Babesia ovis
, it is plausible that sheep in the region could naturally be infected with this tick-borne pathogen.
Methods and results
Blood samples from 81 sheep in the Podrinje and Herzegovina areas were examined by PCR. PCR positivity was found in 38 (46.9%) cases indicating a high number of infected sheep. Mixed infections with
Babesia ovis
and
A.ovis
were observed in 63.3% of cases. A higher number of positive sheep was recorded in the area of Herzegovina. Phylogenetic analysis of the
gltA
,
groEL
, and
msp4
genes of
A. ovis
revealed numerous genotypes and significant genetic variability. This diversity was not related to geographic origin, tick-borne infection status, or sheep breeding practices in Podrinje and Herzegovina.
Conclusions
The data obtained in this study suggest that the emergence of new genotypes and the high genetic variability of
A. ovis
are driven by specific local and micro-environmental factors.</description><identifier>ISSN: 0301-4851</identifier><identifier>ISSN: 1573-4978</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-024-09869-9</identifier><identifier>PMID: 39182201</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Anaplasma ovis ; Anaplasma ovis - genetics ; Anaplasma ovis - isolation & purification ; Anaplasmosis ; Anaplasmosis - epidemiology ; Anaplasmosis - microbiology ; anemia ; Animal Anatomy ; Animal Biochemistry ; Animals ; Arachnids ; Babesia - genetics ; Babesia - isolation & purification ; Babesia ovis ; Babesiosis - epidemiology ; Babesiosis - parasitology ; Biomedical and Life Sciences ; blood ; Bosnia and Herzegovina - epidemiology ; Bosnia-Herzegovina ; Environmental factors ; fever ; Genetic diversity ; Genetic variability ; Genetic Variation ; Genotype ; Genotypes ; Histology ; Jaundice ; Life Sciences ; Mediterranean region ; Morphology ; Original Article ; pathogens ; Phylogeny ; provenance ; Sheep ; Sheep - microbiology ; Sheep - parasitology ; Sheep Diseases - epidemiology ; Sheep Diseases - microbiology ; Sheep Diseases - parasitology ; species</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.936-936, Article 936</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>Copyright Springer Nature B.V. Dec 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-7c5812baff66868719c96b359510e3b96993e30d7c074bc757d3413e6d9592b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-024-09869-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-024-09869-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39182201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stevanović, Oliver</creatorcontrib><creatorcontrib>Ilić, Tamara</creatorcontrib><creatorcontrib>Jovanović, Nemanja</creatorcontrib><creatorcontrib>Vejnović, Branislav</creatorcontrib><creatorcontrib>Radalj, Andrea</creatorcontrib><title>High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Ovine anaplasmosis (
sensu stricto
) is a rickettsial blood disease caused by the tick-borne species
Anaplasma ovis
. The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the presence of
Anaplasma ovis
in Bosnia and Herzegovina. However, given the country’s location within the Mediterranean Basin and the recent molecular detection of
Babesia ovis
, it is plausible that sheep in the region could naturally be infected with this tick-borne pathogen.
Methods and results
Blood samples from 81 sheep in the Podrinje and Herzegovina areas were examined by PCR. PCR positivity was found in 38 (46.9%) cases indicating a high number of infected sheep. Mixed infections with
Babesia ovis
and
A.ovis
were observed in 63.3% of cases. A higher number of positive sheep was recorded in the area of Herzegovina. Phylogenetic analysis of the
gltA
,
groEL
, and
msp4
genes of
A. ovis
revealed numerous genotypes and significant genetic variability. This diversity was not related to geographic origin, tick-borne infection status, or sheep breeding practices in Podrinje and Herzegovina.
Conclusions
The data obtained in this study suggest that the emergence of new genotypes and the high genetic variability of
A. ovis
are driven by specific local and micro-environmental factors.</description><subject>Anaplasma ovis</subject><subject>Anaplasma ovis - genetics</subject><subject>Anaplasma ovis - isolation & purification</subject><subject>Anaplasmosis</subject><subject>Anaplasmosis - epidemiology</subject><subject>Anaplasmosis - microbiology</subject><subject>anemia</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>Arachnids</subject><subject>Babesia - genetics</subject><subject>Babesia - isolation & purification</subject><subject>Babesia ovis</subject><subject>Babesiosis - epidemiology</subject><subject>Babesiosis - parasitology</subject><subject>Biomedical and Life Sciences</subject><subject>blood</subject><subject>Bosnia and Herzegovina - epidemiology</subject><subject>Bosnia-Herzegovina</subject><subject>Environmental factors</subject><subject>fever</subject><subject>Genetic diversity</subject><subject>Genetic variability</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Histology</subject><subject>Jaundice</subject><subject>Life Sciences</subject><subject>Mediterranean region</subject><subject>Morphology</subject><subject>Original Article</subject><subject>pathogens</subject><subject>Phylogeny</subject><subject>provenance</subject><subject>Sheep</subject><subject>Sheep - microbiology</subject><subject>Sheep - parasitology</subject><subject>Sheep Diseases - epidemiology</subject><subject>Sheep Diseases - microbiology</subject><subject>Sheep Diseases - parasitology</subject><subject>species</subject><issn>0301-4851</issn><issn>1573-4978</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1vFDEQhi0EIkfCH0gRWaKhWZjx95RJBBxSEA2pLe-u9-Lo1nux7yKFX88eF4iUAqop5nnf0ehh7BThAwLYjxURpGxAqAbIGWroBVugtrJRZN1LtgAJ2Cin8Yi9qfUWABRa_ZodSUInBOCCfVum1Q1fxRy3qeN9uo-lpu0DnwZ-nsNmHeoY-HSfKk-Z15sYN3wo08gvpppT4CH3fBnLz7iamRxO2KshrGt8-ziP2fXnTz8ul83V9y9fL8-vmk5q2ja20w5FG4bBGGecRerItPNKI0TZkiGSUUJvO7Cq7ay2vVQoo-lJk2iVPGbvD72bMt3tYt36MdUurtchx2lXvUQtLQgrzP9RIIuKhHIz-u4ZejvtSp4f2VPGIjraF4oD1ZWp1hIHvylpDOXBI_i9F3_w4mcv_rcXT3Po7LF6146x_xv5I2IG5AGo8yqvYnm6_Y_aXz9OlYA</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Stevanović, Oliver</creator><creator>Ilić, Tamara</creator><creator>Jovanović, Nemanja</creator><creator>Vejnović, Branislav</creator><creator>Radalj, Andrea</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20241201</creationdate><title>High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina</title><author>Stevanović, Oliver ; Ilić, Tamara ; Jovanović, Nemanja ; Vejnović, Branislav ; Radalj, Andrea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-7c5812baff66868719c96b359510e3b96993e30d7c074bc757d3413e6d9592b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anaplasma ovis</topic><topic>Anaplasma ovis - genetics</topic><topic>Anaplasma ovis - isolation & purification</topic><topic>Anaplasmosis</topic><topic>Anaplasmosis - epidemiology</topic><topic>Anaplasmosis - microbiology</topic><topic>anemia</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animals</topic><topic>Arachnids</topic><topic>Babesia - genetics</topic><topic>Babesia - isolation & purification</topic><topic>Babesia ovis</topic><topic>Babesiosis - epidemiology</topic><topic>Babesiosis - parasitology</topic><topic>Biomedical and Life Sciences</topic><topic>blood</topic><topic>Bosnia and Herzegovina - epidemiology</topic><topic>Bosnia-Herzegovina</topic><topic>Environmental factors</topic><topic>fever</topic><topic>Genetic diversity</topic><topic>Genetic variability</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Histology</topic><topic>Jaundice</topic><topic>Life Sciences</topic><topic>Mediterranean region</topic><topic>Morphology</topic><topic>Original Article</topic><topic>pathogens</topic><topic>Phylogeny</topic><topic>provenance</topic><topic>Sheep</topic><topic>Sheep - microbiology</topic><topic>Sheep - parasitology</topic><topic>Sheep Diseases - epidemiology</topic><topic>Sheep Diseases - microbiology</topic><topic>Sheep Diseases - parasitology</topic><topic>species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stevanović, Oliver</creatorcontrib><creatorcontrib>Ilić, Tamara</creatorcontrib><creatorcontrib>Jovanović, Nemanja</creatorcontrib><creatorcontrib>Vejnović, Branislav</creatorcontrib><creatorcontrib>Radalj, Andrea</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stevanović, Oliver</au><au>Ilić, Tamara</au><au>Jovanović, Nemanja</au><au>Vejnović, Branislav</au><au>Radalj, Andrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>936</spage><epage>936</epage><pages>936-936</pages><artnum>936</artnum><issn>0301-4851</issn><issn>1573-4978</issn><eissn>1573-4978</eissn><abstract>Background
Ovine anaplasmosis (
sensu stricto
) is a rickettsial blood disease caused by the tick-borne species
Anaplasma ovis
. The disease is characterized by mild anemia, fever, and icterus. A more severe clinical presentation is possible in non-endemic areas. There is no existing data on the presence of
Anaplasma ovis
in Bosnia and Herzegovina. However, given the country’s location within the Mediterranean Basin and the recent molecular detection of
Babesia ovis
, it is plausible that sheep in the region could naturally be infected with this tick-borne pathogen.
Methods and results
Blood samples from 81 sheep in the Podrinje and Herzegovina areas were examined by PCR. PCR positivity was found in 38 (46.9%) cases indicating a high number of infected sheep. Mixed infections with
Babesia ovis
and
A.ovis
were observed in 63.3% of cases. A higher number of positive sheep was recorded in the area of Herzegovina. Phylogenetic analysis of the
gltA
,
groEL
, and
msp4
genes of
A. ovis
revealed numerous genotypes and significant genetic variability. This diversity was not related to geographic origin, tick-borne infection status, or sheep breeding practices in Podrinje and Herzegovina.
Conclusions
The data obtained in this study suggest that the emergence of new genotypes and the high genetic variability of
A. ovis
are driven by specific local and micro-environmental factors.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39182201</pmid><doi>10.1007/s11033-024-09869-9</doi><tpages>1</tpages></addata></record> |
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| subjects | Anaplasma ovis Anaplasma ovis - genetics Anaplasma ovis - isolation & purification Anaplasmosis Anaplasmosis - epidemiology Anaplasmosis - microbiology anemia Animal Anatomy Animal Biochemistry Animals Arachnids Babesia - genetics Babesia - isolation & purification Babesia ovis Babesiosis - epidemiology Babesiosis - parasitology Biomedical and Life Sciences blood Bosnia and Herzegovina - epidemiology Bosnia-Herzegovina Environmental factors fever Genetic diversity Genetic variability Genetic Variation Genotype Genotypes Histology Jaundice Life Sciences Mediterranean region Morphology Original Article pathogens Phylogeny provenance Sheep Sheep - microbiology Sheep - parasitology Sheep Diseases - epidemiology Sheep Diseases - microbiology Sheep Diseases - parasitology species |
| title | High genetic diversity of Anaplasma ovis in sheep from Bosnia and Herzegovina |
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