Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment
Objectives This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group — GI). It also aimed to compare the findings with th...
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Veröffentlicht in: | Clinical oral investigations 2022, Vol.26 (1), p.287-302 |
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creator | Gabrielli, Ezequiel S. Lima, Augusto R. Francisco, Priscila A. Herrera, Daniel R. de-Jesus-Soares, Adriana Ferraz, Caio C. R. Almeida, José Flávio A. Marciano, Marina A. Gomes, Brenda P. F. A. |
description | Objectives
This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group — GI). It also aimed to compare the findings with those presented by asymptomatic necrotic root canals (asymptomatic group — GII) in the different stages of the endodontic treatment.
Materials and methods
Microbiological samples were collected from 20 root canals, including purulent collection from acute apical abscesses, before and after chemo-mechanical preparation (CMP) preparation (CMP) with chlorhexidine gel 2% and after 30 days of intracanal medication (ICM) with (Ca[OH]
2
) + chlorhexidine. The culture method was used to evaluate the efficacy of CMP and ICM. Nested PCR was used to detect the presence of 17 specific bacteria. Levels of LPS were measured by using limulus amebocyte lysate, whereas enzyme-linked immunosorbent assay was used for the quantification of LTA.
Results
CMP was effective in reducing the microbial load in both groups (
P
|
doi_str_mv | 10.1007/s00784-021-03998-2 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2540520661</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2622860509</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-bb2c91df325f5919725c994a4fdfee5d35699b2e6ee502a2d0c77b648688dfc3</originalsourceid><addsrcrecordid>eNp9kc1u1TAQhS1ERUvhBVggS2xYkOL_xEt0xZ9UqZvuLccet66SONi-le7b8WiY3AIVi27s8cw350g-CL2h5IIS0n8s7RhERxjtCNd66NgzdEYFVx3ve_p8q1mn9EBP0ctS7gihQvX8BTrlglJBCT1DP3dpXm22Nd4DtoudDiUWnAIerauQo52wS0uFpX7AE9zDtA2nuKY1NdY6d2tz9FDast_6FaK7TdFh66LHccHlMK81zc3CbZB93IDFJ98MWhmXAK7GtDStin0MAXLzxaXaG9hsH8E1g61zG79CJ8FOBV4_3Ofo-svn69237vLq6_fdp8vO8V7WbhyZ09QHzmSQmuqeSae1sCL4ACA9l0rrkYFqD8Is88T1_ajEoIbBB8fP0fuj7JrTjz2UauZYHEyTXSDti2FSEMmIUrSh7_5D79I-t69tlGJsUEQS3Sh2pFxOpWQIZs1xtvlgKDG_4zXHeE2L12zxGtaW3j5I78cZ_N-VP3k2gB-B0kbLDeR_3k_I_gKjjLYY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2622860509</pqid></control><display><type>article</type><title>Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Gabrielli, Ezequiel S. ; Lima, Augusto R. ; Francisco, Priscila A. ; Herrera, Daniel R. ; de-Jesus-Soares, Adriana ; Ferraz, Caio C. R. ; Almeida, José Flávio A. ; Marciano, Marina A. ; Gomes, Brenda P. F. A.</creator><creatorcontrib>Gabrielli, Ezequiel S. ; Lima, Augusto R. ; Francisco, Priscila A. ; Herrera, Daniel R. ; de-Jesus-Soares, Adriana ; Ferraz, Caio C. R. ; Almeida, José Flávio A. ; Marciano, Marina A. ; Gomes, Brenda P. F. A.</creatorcontrib><description>Objectives
This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group — GI). It also aimed to compare the findings with those presented by asymptomatic necrotic root canals (asymptomatic group — GII) in the different stages of the endodontic treatment.
Materials and methods
Microbiological samples were collected from 20 root canals, including purulent collection from acute apical abscesses, before and after chemo-mechanical preparation (CMP) preparation (CMP) with chlorhexidine gel 2% and after 30 days of intracanal medication (ICM) with (Ca[OH]
2
) + chlorhexidine. The culture method was used to evaluate the efficacy of CMP and ICM. Nested PCR was used to detect the presence of 17 specific bacteria. Levels of LPS were measured by using limulus amebocyte lysate, whereas enzyme-linked immunosorbent assay was used for the quantification of LTA.
Results
CMP was effective in reducing the microbial load in both groups (
P
< 0.05). LPS levels were higher in GI than in GII (
P
< 0.05). There was a significant reduction in the LPS levels after CMP and ICM (
P
< 0.05) in GI and GII. LTA levels were significantly reduced in GI after ICM and in GII after CMP and ICM (both
P
< 0.05).
Fusobacterium nucleatum
and
Enterococcus faecalis
were frequently identified in both groups, alone or in combination with each other.
Conclusion
Different species were detected in all stages of the endodontic treatment. CMP was able to reduce bacterial content and the levels of LPS, but not of LTA in the symptomatic group. High levels of LPS were correlated with spontaneous pain and pain to percussion in the symptomatic group.
Clinical relevance
This clinical study showed that chemo-mechanical preparation was able to reduce bacterial load and levels of LPS, but not of LTA in the symptomatic group. Elevated levels of LPS were correlated with the presence of symptomatology.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-021-03998-2</identifier><identifier>PMID: 34114101</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abscesses ; Asymptomatic ; Bacteria ; Chlorhexidine ; Comparative analysis ; Dental Pulp Cavity ; Dentistry ; Endodontics ; Enzyme-linked immunosorbent assay ; Humans ; Lipopolysaccharides ; Lipoteichoic acid ; Medicine ; Original Article ; Pain ; Periapical Periodontitis ; Root Canal Irrigants ; Root canals ; Teichoic Acids</subject><ispartof>Clinical oral investigations, 2022, Vol.26 (1), p.287-302</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-bb2c91df325f5919725c994a4fdfee5d35699b2e6ee502a2d0c77b648688dfc3</citedby><cites>FETCH-LOGICAL-c375t-bb2c91df325f5919725c994a4fdfee5d35699b2e6ee502a2d0c77b648688dfc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-021-03998-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-021-03998-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34114101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gabrielli, Ezequiel S.</creatorcontrib><creatorcontrib>Lima, Augusto R.</creatorcontrib><creatorcontrib>Francisco, Priscila A.</creatorcontrib><creatorcontrib>Herrera, Daniel R.</creatorcontrib><creatorcontrib>de-Jesus-Soares, Adriana</creatorcontrib><creatorcontrib>Ferraz, Caio C. R.</creatorcontrib><creatorcontrib>Almeida, José Flávio A.</creatorcontrib><creatorcontrib>Marciano, Marina A.</creatorcontrib><creatorcontrib>Gomes, Brenda P. F. A.</creatorcontrib><title>Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group — GI). It also aimed to compare the findings with those presented by asymptomatic necrotic root canals (asymptomatic group — GII) in the different stages of the endodontic treatment.
Materials and methods
Microbiological samples were collected from 20 root canals, including purulent collection from acute apical abscesses, before and after chemo-mechanical preparation (CMP) preparation (CMP) with chlorhexidine gel 2% and after 30 days of intracanal medication (ICM) with (Ca[OH]
2
) + chlorhexidine. The culture method was used to evaluate the efficacy of CMP and ICM. Nested PCR was used to detect the presence of 17 specific bacteria. Levels of LPS were measured by using limulus amebocyte lysate, whereas enzyme-linked immunosorbent assay was used for the quantification of LTA.
Results
CMP was effective in reducing the microbial load in both groups (
P
< 0.05). LPS levels were higher in GI than in GII (
P
< 0.05). There was a significant reduction in the LPS levels after CMP and ICM (
P
< 0.05) in GI and GII. LTA levels were significantly reduced in GI after ICM and in GII after CMP and ICM (both
P
< 0.05).
Fusobacterium nucleatum
and
Enterococcus faecalis
were frequently identified in both groups, alone or in combination with each other.
Conclusion
Different species were detected in all stages of the endodontic treatment. CMP was able to reduce bacterial content and the levels of LPS, but not of LTA in the symptomatic group. High levels of LPS were correlated with spontaneous pain and pain to percussion in the symptomatic group.
Clinical relevance
This clinical study showed that chemo-mechanical preparation was able to reduce bacterial load and levels of LPS, but not of LTA in the symptomatic group. Elevated levels of LPS were correlated with the presence of symptomatology.</description><subject>Abscesses</subject><subject>Asymptomatic</subject><subject>Bacteria</subject><subject>Chlorhexidine</subject><subject>Comparative analysis</subject><subject>Dental Pulp Cavity</subject><subject>Dentistry</subject><subject>Endodontics</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Humans</subject><subject>Lipopolysaccharides</subject><subject>Lipoteichoic acid</subject><subject>Medicine</subject><subject>Original Article</subject><subject>Pain</subject><subject>Periapical Periodontitis</subject><subject>Root Canal Irrigants</subject><subject>Root canals</subject><subject>Teichoic Acids</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1TAQhS1ERUvhBVggS2xYkOL_xEt0xZ9UqZvuLccet66SONi-le7b8WiY3AIVi27s8cw350g-CL2h5IIS0n8s7RhERxjtCNd66NgzdEYFVx3ve_p8q1mn9EBP0ctS7gihQvX8BTrlglJBCT1DP3dpXm22Nd4DtoudDiUWnAIerauQo52wS0uFpX7AE9zDtA2nuKY1NdY6d2tz9FDast_6FaK7TdFh66LHccHlMK81zc3CbZB93IDFJ98MWhmXAK7GtDStin0MAXLzxaXaG9hsH8E1g61zG79CJ8FOBV4_3Ofo-svn69237vLq6_fdp8vO8V7WbhyZ09QHzmSQmuqeSae1sCL4ACA9l0rrkYFqD8Is88T1_ajEoIbBB8fP0fuj7JrTjz2UauZYHEyTXSDti2FSEMmIUrSh7_5D79I-t69tlGJsUEQS3Sh2pFxOpWQIZs1xtvlgKDG_4zXHeE2L12zxGtaW3j5I78cZ_N-VP3k2gB-B0kbLDeR_3k_I_gKjjLYY</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Gabrielli, Ezequiel S.</creator><creator>Lima, Augusto R.</creator><creator>Francisco, Priscila A.</creator><creator>Herrera, Daniel R.</creator><creator>de-Jesus-Soares, Adriana</creator><creator>Ferraz, Caio C. R.</creator><creator>Almeida, José Flávio A.</creator><creator>Marciano, Marina A.</creator><creator>Gomes, Brenda P. F. A.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>2022</creationdate><title>Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment</title><author>Gabrielli, Ezequiel S. ; Lima, Augusto R. ; Francisco, Priscila A. ; Herrera, Daniel R. ; de-Jesus-Soares, Adriana ; Ferraz, Caio C. R. ; Almeida, José Flávio A. ; Marciano, Marina A. ; Gomes, Brenda P. F. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-bb2c91df325f5919725c994a4fdfee5d35699b2e6ee502a2d0c77b648688dfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abscesses</topic><topic>Asymptomatic</topic><topic>Bacteria</topic><topic>Chlorhexidine</topic><topic>Comparative analysis</topic><topic>Dental Pulp Cavity</topic><topic>Dentistry</topic><topic>Endodontics</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Humans</topic><topic>Lipopolysaccharides</topic><topic>Lipoteichoic acid</topic><topic>Medicine</topic><topic>Original Article</topic><topic>Pain</topic><topic>Periapical Periodontitis</topic><topic>Root Canal Irrigants</topic><topic>Root canals</topic><topic>Teichoic Acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gabrielli, Ezequiel S.</creatorcontrib><creatorcontrib>Lima, Augusto R.</creatorcontrib><creatorcontrib>Francisco, Priscila A.</creatorcontrib><creatorcontrib>Herrera, Daniel R.</creatorcontrib><creatorcontrib>de-Jesus-Soares, Adriana</creatorcontrib><creatorcontrib>Ferraz, Caio C. R.</creatorcontrib><creatorcontrib>Almeida, José Flávio A.</creatorcontrib><creatorcontrib>Marciano, Marina A.</creatorcontrib><creatorcontrib>Gomes, Brenda P. F. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gabrielli, Ezequiel S.</au><au>Lima, Augusto R.</au><au>Francisco, Priscila A.</au><au>Herrera, Daniel R.</au><au>de-Jesus-Soares, Adriana</au><au>Ferraz, Caio C. R.</au><au>Almeida, José Flávio A.</au><au>Marciano, Marina A.</au><au>Gomes, Brenda P. F. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2022</date><risdate>2022</risdate><volume>26</volume><issue>1</issue><spage>287</spage><epage>302</epage><pages>287-302</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group — GI). It also aimed to compare the findings with those presented by asymptomatic necrotic root canals (asymptomatic group — GII) in the different stages of the endodontic treatment.
Materials and methods
Microbiological samples were collected from 20 root canals, including purulent collection from acute apical abscesses, before and after chemo-mechanical preparation (CMP) preparation (CMP) with chlorhexidine gel 2% and after 30 days of intracanal medication (ICM) with (Ca[OH]
2
) + chlorhexidine. The culture method was used to evaluate the efficacy of CMP and ICM. Nested PCR was used to detect the presence of 17 specific bacteria. Levels of LPS were measured by using limulus amebocyte lysate, whereas enzyme-linked immunosorbent assay was used for the quantification of LTA.
Results
CMP was effective in reducing the microbial load in both groups (
P
< 0.05). LPS levels were higher in GI than in GII (
P
< 0.05). There was a significant reduction in the LPS levels after CMP and ICM (
P
< 0.05) in GI and GII. LTA levels were significantly reduced in GI after ICM and in GII after CMP and ICM (both
P
< 0.05).
Fusobacterium nucleatum
and
Enterococcus faecalis
were frequently identified in both groups, alone or in combination with each other.
Conclusion
Different species were detected in all stages of the endodontic treatment. CMP was able to reduce bacterial content and the levels of LPS, but not of LTA in the symptomatic group. High levels of LPS were correlated with spontaneous pain and pain to percussion in the symptomatic group.
Clinical relevance
This clinical study showed that chemo-mechanical preparation was able to reduce bacterial load and levels of LPS, but not of LTA in the symptomatic group. Elevated levels of LPS were correlated with the presence of symptomatology.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34114101</pmid><doi>10.1007/s00784-021-03998-2</doi><tpages>16</tpages></addata></record> |
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source | MEDLINE; Springer Nature - Complete Springer Journals |
subjects | Abscesses Asymptomatic Bacteria Chlorhexidine Comparative analysis Dental Pulp Cavity Dentistry Endodontics Enzyme-linked immunosorbent assay Humans Lipopolysaccharides Lipoteichoic acid Medicine Original Article Pain Periapical Periodontitis Root Canal Irrigants Root canals Teichoic Acids |
title | Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment |
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