Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment

Objectives This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group — GI). It also aimed to compare the findings with th...

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Veröffentlicht in:Clinical oral investigations 2022, Vol.26 (1), p.287-302
Hauptverfasser: Gabrielli, Ezequiel S., Lima, Augusto R., Francisco, Priscila A., Herrera, Daniel R., de-Jesus-Soares, Adriana, Ferraz, Caio C. R., Almeida, José Flávio A., Marciano, Marina A., Gomes, Brenda P. F. A.
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Sprache:eng
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Zusammenfassung:Objectives This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group — GI). It also aimed to compare the findings with those presented by asymptomatic necrotic root canals (asymptomatic group — GII) in the different stages of the endodontic treatment. Materials and methods Microbiological samples were collected from 20 root canals, including purulent collection from acute apical abscesses, before and after chemo-mechanical preparation (CMP) preparation (CMP) with chlorhexidine gel 2% and after 30 days of intracanal medication (ICM) with (Ca[OH] 2 ) + chlorhexidine. The culture method was used to evaluate the efficacy of CMP and ICM. Nested PCR was used to detect the presence of 17 specific bacteria. Levels of LPS were measured by using limulus amebocyte lysate, whereas enzyme-linked immunosorbent assay was used for the quantification of LTA. Results CMP was effective in reducing the microbial load in both groups ( P  
ISSN:1432-6981
1436-3771
DOI:10.1007/s00784-021-03998-2