Purification of recombinant hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A expressed in Escherichia coli and its application for the specific determination of hyaluronan concentration
Hyaluronan (HA) lyase of Streptococcus pyogenes bacteriophage was expressed in Escherichia coli and purified to homogeneity by immobilized metal affinity chromatography (IMAC). Unlike most bacterial HA lyases, the phage enzyme specifically cleaved HA to unsaturated oligosaccharides which has an opti...
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creator | Yang, Pei-Fen Lee, Cheng-Kang |
description | Hyaluronan (HA) lyase of
Streptococcus pyogenes bacteriophage was expressed in
Escherichia coli and purified to homogeneity by immobilized metal affinity chromatography (IMAC). Unlike most bacterial HA lyases, the phage enzyme specifically cleaved HA to unsaturated oligosaccharides which has an optimum absorption at 232
nm. The absorbance of the digestion product reached a limiting value as reaction time increased. The limiting absorbance showed linearity in the range of concentrations 0.05–0.5
mg/mL. Based on this fact, a specific, simple, easy to apply, low cost, and fast enough method was developed for routine determination of HA concentration of a microbial HA production process. This phage HA lyase-based limiting absorbance method has same accuracy and sensitivity as conventional carbazole method for the determination of HA produced from
Streptococcus zooepidemicus cultivation. |
doi_str_mv | 10.1016/j.carbpol.2005.12.037 |
format | Article |
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Streptococcus pyogenes bacteriophage was expressed in
Escherichia coli and purified to homogeneity by immobilized metal affinity chromatography (IMAC). Unlike most bacterial HA lyases, the phage enzyme specifically cleaved HA to unsaturated oligosaccharides which has an optimum absorption at 232
nm. The absorbance of the digestion product reached a limiting value as reaction time increased. The limiting absorbance showed linearity in the range of concentrations 0.05–0.5
mg/mL. Based on this fact, a specific, simple, easy to apply, low cost, and fast enough method was developed for routine determination of HA concentration of a microbial HA production process. This phage HA lyase-based limiting absorbance method has same accuracy and sensitivity as conventional carbazole method for the determination of HA produced from
Streptococcus zooepidemicus cultivation.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2005.12.037</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Biotechnology ; Carbazole method ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Hyaluronan ; Hyaluronan determination ; Limiting absorbance ; Phage ha ; Phage hyaluronan lyase ; Streptococcus pyogenes</subject><ispartof>Carbohydrate polymers, 2006-07, Vol.65 (2), p.159-164</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-c32fc6cfd800a27b3b33a7ed60f2e47bd7980517a4192b484ccbb6316aae54913</citedby><cites>FETCH-LOGICAL-c370t-c32fc6cfd800a27b3b33a7ed60f2e47bd7980517a4192b484ccbb6316aae54913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2005.12.037$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18013464$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Pei-Fen</creatorcontrib><creatorcontrib>Lee, Cheng-Kang</creatorcontrib><title>Purification of recombinant hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A expressed in Escherichia coli and its application for the specific determination of hyaluronan concentration</title><title>Carbohydrate polymers</title><description>Hyaluronan (HA) lyase of
Streptococcus pyogenes bacteriophage was expressed in
Escherichia coli and purified to homogeneity by immobilized metal affinity chromatography (IMAC). Unlike most bacterial HA lyases, the phage enzyme specifically cleaved HA to unsaturated oligosaccharides which has an optimum absorption at 232
nm. The absorbance of the digestion product reached a limiting value as reaction time increased. The limiting absorbance showed linearity in the range of concentrations 0.05–0.5
mg/mL. Based on this fact, a specific, simple, easy to apply, low cost, and fast enough method was developed for routine determination of HA concentration of a microbial HA production process. This phage HA lyase-based limiting absorbance method has same accuracy and sensitivity as conventional carbazole method for the determination of HA produced from
Streptococcus zooepidemicus cultivation.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbazole method</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hyaluronan</subject><subject>Hyaluronan determination</subject><subject>Limiting absorbance</subject><subject>Phage ha</subject><subject>Phage hyaluronan lyase</subject><subject>Streptococcus pyogenes</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhiMEEkvbR0DyBW6b2ok3Tk6oqgqtVAmk0rM1mUwar7K2sR3UfUMeCy-7FdzwwR55_pn57a8o3gteCi6ay22JEHrv5rLifFOKquS1elWsRKu6tailfF2suJBy3TZCvS3exbjleTWCr4pf35ZgRoOQjLPMjSwQul1vLNjEpj3MS3A5ZvMeIh3yDymQTw4d4hKZ37snshRZD5goGOcneCJ2K2XbXTF69oFipIEZy24iTlmBkwGGbjYMbL5PkYH384uB0QWWJmLREx5ssYFy21228-LvH0_oLJJN4U_uvHgzwhzp4nSeFY-fb75f367vv365u766X2OteMp7NWKD49ByDpXq676uQdHQ8LEiqfpBdS3fCAVSdFUvW4nY900tGgDayE7UZ8XHY18f3I-FYtI7E5HmGSy5JWrRqbbtuMrCzVGIwcUYaNQ-mB2EvRZcH7jprT5x0wduWlQ6c8t1H04DICLMYwCLJv4tbnlG2sis-3TUUX7tT0NBRzSUf2QwmWHSgzP_mfQbEH-3SQ</recordid><startdate>20060725</startdate><enddate>20060725</enddate><creator>Yang, Pei-Fen</creator><creator>Lee, Cheng-Kang</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20060725</creationdate><title>Purification of recombinant hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A expressed in Escherichia coli and its application for the specific determination of hyaluronan concentration</title><author>Yang, Pei-Fen ; Lee, Cheng-Kang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-c32fc6cfd800a27b3b33a7ed60f2e47bd7980517a4192b484ccbb6316aae54913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbazole method</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hyaluronan</topic><topic>Hyaluronan determination</topic><topic>Limiting absorbance</topic><topic>Phage ha</topic><topic>Phage hyaluronan lyase</topic><topic>Streptococcus pyogenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Pei-Fen</creatorcontrib><creatorcontrib>Lee, Cheng-Kang</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Pei-Fen</au><au>Lee, Cheng-Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of recombinant hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A expressed in Escherichia coli and its application for the specific determination of hyaluronan concentration</atitle><jtitle>Carbohydrate polymers</jtitle><date>2006-07-25</date><risdate>2006</risdate><volume>65</volume><issue>2</issue><spage>159</spage><epage>164</epage><pages>159-164</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>Hyaluronan (HA) lyase of
Streptococcus pyogenes bacteriophage was expressed in
Escherichia coli and purified to homogeneity by immobilized metal affinity chromatography (IMAC). Unlike most bacterial HA lyases, the phage enzyme specifically cleaved HA to unsaturated oligosaccharides which has an optimum absorption at 232
nm. The absorbance of the digestion product reached a limiting value as reaction time increased. The limiting absorbance showed linearity in the range of concentrations 0.05–0.5
mg/mL. Based on this fact, a specific, simple, easy to apply, low cost, and fast enough method was developed for routine determination of HA concentration of a microbial HA production process. This phage HA lyase-based limiting absorbance method has same accuracy and sensitivity as conventional carbazole method for the determination of HA produced from
Streptococcus zooepidemicus cultivation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2005.12.037</doi><tpages>6</tpages></addata></record> |
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language | eng |
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source | Elsevier ScienceDirect Journals Complete |
subjects | Biological and medical sciences Biotechnology Carbazole method Escherichia coli Fundamental and applied biological sciences. Psychology Hyaluronan Hyaluronan determination Limiting absorbance Phage ha Phage hyaluronan lyase Streptococcus pyogenes |
title | Purification of recombinant hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A expressed in Escherichia coli and its application for the specific determination of hyaluronan concentration |
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