Purification of recombinant hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A expressed in Escherichia coli and its application for the specific determination of hyaluronan concentration

Hyaluronan (HA) lyase of Streptococcus pyogenes bacteriophage was expressed in Escherichia coli and purified to homogeneity by immobilized metal affinity chromatography (IMAC). Unlike most bacterial HA lyases, the phage enzyme specifically cleaved HA to unsaturated oligosaccharides which has an opti...

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Veröffentlicht in:	Carbohydrate polymers 2006-07, Vol.65 (2), p.159-164
Hauptverfasser:	Yang, Pei-Fen, Lee, Cheng-Kang
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Carbazole method Escherichia coli Fundamental and applied biological sciences. Psychology Hyaluronan Hyaluronan determination Limiting absorbance Phage ha Phage hyaluronan lyase Streptococcus pyogenes
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