Engineering fatty acid synthases for directed polyketide production
In vitro and in silico analysis enables the rational design of fatty acid synthase (FAS)-mediated pathways for the compartmentalized production of desirable fatty acids and a polyketide lactone. In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids a...
Gespeichert in:
Veröffentlicht in: | Nature chemical biology 2017-04, Vol.13 (4), p.363-365 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | In vitro
and
in silico
analysis enables the rational design of fatty acid synthase (FAS)-mediated pathways for the compartmentalized production of desirable fatty acids and a polyketide lactone.
In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined
in vitro
and
in silico
approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds. |
---|---|
ISSN: | 1552-4450 1552-4469 |
DOI: | 10.1038/nchembio.2314 |