Engineering fatty acid synthases for directed polyketide production

In vitro and in silico analysis enables the rational design of fatty acid synthase (FAS)-mediated pathways for the compartmentalized production of desirable fatty acids and a polyketide lactone. In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids a...

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Veröffentlicht in:Nature chemical biology 2017-04, Vol.13 (4), p.363-365
Hauptverfasser: Gajewski, Jan, Buelens, Floris, Serdjukow, Sascha, Janßen, Melanie, Cortina, Niña, Grubmüller, Helmut, Grininger, Martin
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Sprache:eng
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Zusammenfassung:In vitro and in silico analysis enables the rational design of fatty acid synthase (FAS)-mediated pathways for the compartmentalized production of desirable fatty acids and a polyketide lactone. In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds.
ISSN:1552-4450
1552-4469
DOI:10.1038/nchembio.2314