PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications
N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora . A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed....
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Veröffentlicht in: | Applied biochemistry and microbiology 2017-03, Vol.53 (2), p.165-172 |
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Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from
Erwinia carotovora
. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of the enzyme with a 25-fold excess of reagent relative to the enzyme tetramer. The modified L-asparaginase was isolated from the reaction mixture by gel filtration on Sepharose CL-6B. The purified bioconjugate did not contain PEG unbound to the protein, demonstrated high catalytic activity, and exhibited antiproliferative action on cell cultures. |
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ISSN: | 0003-6838 1608-3024 |
DOI: | 10.1134/S0003683817020119 |