Endophytic Bacterial Population in Philippine Sugarcane Cultivars and Isolation of Nitrogen-fixing Strains

The number of endophytic bacteria in the stem of sugarcane cultivars collected from different sites in the Philippines ranged from 104 to 109 cells ml-1 juice. Bacterial population was correlated with the sugar content (% Brix) of the juice and varied depending on the cultivar and sampling site. Twe...

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Veröffentlicht in:Microbes and Environments 2000, Vol.15(4), pp.209-216
Hauptverfasser: ASIS, CONSTANCIO A. JR, KUBOTA, MASATSUGU, CHEBOTAR, VLADIMIR K., OHTA, HIROYUKI, ARIMA, YASUHIRO, NISHIYAMA, KOUSHI, TSUCHIYA, KEN-ICHI, AKAO, SHOICHIRO
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Sprache:eng
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Zusammenfassung:The number of endophytic bacteria in the stem of sugarcane cultivars collected from different sites in the Philippines ranged from 104 to 109 cells ml-1 juice. Bacterial population was correlated with the sugar content (% Brix) of the juice and varied depending on the cultivar and sampling site. Twenty-eight isolates had a positive reaction in the acetylene reduction assay (ARA). Presumptive tests indicated that 15 isolates were putative strains of Acetobacter diazotrophicus, 5 isolates showed similar characteristics to those of Herbaspirillum seropedicae, and 8 isolates consisted of Herbaspirillum rubribalbicans-like strains. This study confirmed the existence of N2-fixing endophytic bacteria in sugarcane cultivars in the Philippines.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.2000.209