DNA polymerase activities in healthy and cauliflower mosaic virus-infected turnip (Brassica rapa) plants
Turnip plants contain three types of DNA polymerase: DNA polymerase-α, DNA polymerase-β and DNA polymerase-γ, the latter characterized by its use of poly-A. oligo-dT as a template-primer; polymerase-α and -γ are soluble, whereas polymerase-β is bound to chromatin. DNA polymerases-α and γ have been p...
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Veröffentlicht in: | Annals of botany 1986, Vol.57 (1), p.81-89 |
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Sprache: | eng |
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Zusammenfassung: | Turnip plants contain three types of DNA polymerase: DNA polymerase-α, DNA polymerase-β and DNA polymerase-γ, the latter characterized by its use of poly-A. oligo-dT as a template-primer; polymerase-α and -γ are soluble, whereas polymerase-β is bound to chromatin. DNA polymerases-α and γ have been purified by (NH4)2SO4 precipitation followed by DEAE-cellulose chromatography; DNA polymerase-β has been purified by solubilization from chromatin followed by DEAE-cellulose chromatography. All three enzymes have been characterized in terms of pH and ionic requirements and response to inhibitors. Infection of turnip plants with cauliflower mosaic virus (CaMV) does not lead to any changes in the chromatographic behaviour or in the general properties of the enzymes, except for a slight reduction in the degree of inhibition by phosphate of DNA polymerase-β. The data are discussed in relation to the possible identify of CaMV replicase in infected plants. |
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ISSN: | 0305-7364 1095-8290 |
DOI: | 10.1093/oxfordjournals.aob.a087096 |