DNA polymerase activities in healthy and cauliflower mosaic virus-infected turnip (Brassica rapa) plants
Turnip plants contain three types of DNA polymerase: DNA polymerase-α, DNA polymerase-β and DNA polymerase-γ, the latter characterized by its use of poly-A. oligo-dT as a template-primer; polymerase-α and -γ are soluble, whereas polymerase-β is bound to chromatin. DNA polymerases-α and γ have been p...
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| Veröffentlicht in: | Annals of botany 1986, Vol.57 (1), p.81-89 |
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| creator | Dunham, V.L Bryant, J.A |
| description | Turnip plants contain three types of DNA polymerase: DNA polymerase-α, DNA polymerase-β and DNA polymerase-γ, the latter characterized by its use of poly-A. oligo-dT as a template-primer; polymerase-α and -γ are soluble, whereas polymerase-β is bound to chromatin. DNA polymerases-α and γ have been purified by (NH4)2SO4 precipitation followed by DEAE-cellulose chromatography; DNA polymerase-β has been purified by solubilization from chromatin followed by DEAE-cellulose chromatography. All three enzymes have been characterized in terms of pH and ionic requirements and response to inhibitors. Infection of turnip plants with cauliflower mosaic virus (CaMV) does not lead to any changes in the chromatographic behaviour or in the general properties of the enzymes, except for a slight reduction in the degree of inhibition by phosphate of DNA polymerase-β. The data are discussed in relation to the possible identify of CaMV replicase in infected plants. |
| doi_str_mv | 10.1093/oxfordjournals.aob.a087096 |
| format | Article |
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DNA polymerases-α and γ have been purified by (NH4)2SO4 precipitation followed by DEAE-cellulose chromatography; DNA polymerase-β has been purified by solubilization from chromatin followed by DEAE-cellulose chromatography. All three enzymes have been characterized in terms of pH and ionic requirements and response to inhibitors. Infection of turnip plants with cauliflower mosaic virus (CaMV) does not lead to any changes in the chromatographic behaviour or in the general properties of the enzymes, except for a slight reduction in the degree of inhibition by phosphate of DNA polymerase-β. The data are discussed in relation to the possible identify of CaMV replicase in infected plants.</description><identifier>ISSN: 0305-7364</identifier><identifier>EISSN: 1095-8290</identifier><identifier>DOI: 10.1093/oxfordjournals.aob.a087096</identifier><identifier>CODEN: ANBOA4</identifier><language>eng</language><publisher>London: Oxford University Press</publisher><subject>Biochemistry ; Biological and medical sciences ; Brassica rapa ; Brassica rapa subsp. campestris ; Cauliflower mosaic virus ; cauliflower mosaic virus replicase ; Caulimovirus ; Chromatography ; DNA ; DNA biosynthesis ; DNA polymerase ; DNA replication ; DNA-directed DNA polymerase ; enzyme activity ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Pathology. Damages, economic importance ; Phosphates ; Phytopathology. Animal pests. Plant and forest protection ; Plant cells ; Plant viruses and viroids ; Plants ; Turnip ; Turnips</subject><ispartof>Annals of botany, 1986, Vol.57 (1), p.81-89</ispartof><rights>1986 Annals of Botany Company</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-8c63fd35bc8c689a01b08b4b51b867e93af02be789e4d991c3174a891b9c48d83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42757568$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42757568$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,4024,27923,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8809068$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Dunham, V.L</creatorcontrib><creatorcontrib>Bryant, J.A</creatorcontrib><title>DNA polymerase activities in healthy and cauliflower mosaic virus-infected turnip (Brassica rapa) plants</title><title>Annals of botany</title><description>Turnip plants contain three types of DNA polymerase: DNA polymerase-α, DNA polymerase-β and DNA polymerase-γ, the latter characterized by its use of poly-A. oligo-dT as a template-primer; polymerase-α and -γ are soluble, whereas polymerase-β is bound to chromatin. DNA polymerases-α and γ have been purified by (NH4)2SO4 precipitation followed by DEAE-cellulose chromatography; DNA polymerase-β has been purified by solubilization from chromatin followed by DEAE-cellulose chromatography. All three enzymes have been characterized in terms of pH and ionic requirements and response to inhibitors. Infection of turnip plants with cauliflower mosaic virus (CaMV) does not lead to any changes in the chromatographic behaviour or in the general properties of the enzymes, except for a slight reduction in the degree of inhibition by phosphate of DNA polymerase-β. The data are discussed in relation to the possible identify of CaMV replicase in infected plants.</description><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Brassica rapa</subject><subject>Brassica rapa subsp. campestris</subject><subject>Cauliflower mosaic virus</subject><subject>cauliflower mosaic virus replicase</subject><subject>Caulimovirus</subject><subject>Chromatography</subject><subject>DNA</subject><subject>DNA biosynthesis</subject><subject>DNA polymerase</subject><subject>DNA replication</subject><subject>DNA-directed DNA polymerase</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Pathology. Damages, economic importance</subject><subject>Phosphates</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant cells</subject><subject>Plant viruses and viroids</subject><subject>Plants</subject><subject>Turnip</subject><subject>Turnips</subject><issn>0305-7364</issn><issn>1095-8290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNpVkFtv1DAQhSMEEkvhJyAshBA8ZLFjxxfe2nIpYlkeShHixZo4DuttNg62U7r_HpesVuJpRjrfnDk6RfGc4CXBir7xt50P7dZPYYA-LsE3S8BSYMXvFYtM1KWsFL5fLDDFdSkoZw-LRzFuMcYVV2RRbN6tT9Ho-_3OBogWgUnuxiVnI3ID2ljo02aPYGiRgal3Xe__2IB2PoIz6MaFKZZu6KxJtkUpp3AjenWWnaIzgAKM8BqNPQwpPi4edDmifXKYJ8XVh_ffzi_K1dePn85PV6VhWKRSGk67ltaNyZtUgEmDZcOamjSSC6sodLhqrJDKslYpYigRDKQijTJMtpKeFC9n3zH435ONSe9cNLbPIayfoias5pRQlsG3M2iCjzHYTo_B7SDsNcH6rlz9f7k6l6sP5ebjF4cvEA30XYDBuHh0kBIrzO_CPJ2xbUw-HGVWiVrU__Ry1l1M9vaoQ7jWXFBR64sfP_Xquzpbf75c6y-ZfzbzHXgNv0J-eXVZYUIxYYzXvKJ_AfgRpKk</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Dunham, V.L</creator><creator>Bryant, J.A</creator><general>Oxford University Press</general><general>Academic Press Inc</general><general>Academic Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>1986</creationdate><title>DNA polymerase activities in healthy and cauliflower mosaic virus-infected turnip (Brassica rapa) plants</title><author>Dunham, V.L ; Bryant, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-8c63fd35bc8c689a01b08b4b51b867e93af02be789e4d991c3174a891b9c48d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Brassica rapa</topic><topic>Brassica rapa subsp. campestris</topic><topic>Cauliflower mosaic virus</topic><topic>cauliflower mosaic virus replicase</topic><topic>Caulimovirus</topic><topic>Chromatography</topic><topic>DNA</topic><topic>DNA biosynthesis</topic><topic>DNA polymerase</topic><topic>DNA replication</topic><topic>DNA-directed DNA polymerase</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Pathology. Damages, economic importance</topic><topic>Phosphates</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant cells</topic><topic>Plant viruses and viroids</topic><topic>Plants</topic><topic>Turnip</topic><topic>Turnips</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dunham, V.L</creatorcontrib><creatorcontrib>Bryant, J.A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Annals of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dunham, V.L</au><au>Bryant, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA polymerase activities in healthy and cauliflower mosaic virus-infected turnip (Brassica rapa) plants</atitle><jtitle>Annals of botany</jtitle><date>1986</date><risdate>1986</risdate><volume>57</volume><issue>1</issue><spage>81</spage><epage>89</epage><pages>81-89</pages><issn>0305-7364</issn><eissn>1095-8290</eissn><coden>ANBOA4</coden><abstract>Turnip plants contain three types of DNA polymerase: DNA polymerase-α, DNA polymerase-β and DNA polymerase-γ, the latter characterized by its use of poly-A. oligo-dT as a template-primer; polymerase-α and -γ are soluble, whereas polymerase-β is bound to chromatin. DNA polymerases-α and γ have been purified by (NH4)2SO4 precipitation followed by DEAE-cellulose chromatography; DNA polymerase-β has been purified by solubilization from chromatin followed by DEAE-cellulose chromatography. All three enzymes have been characterized in terms of pH and ionic requirements and response to inhibitors. Infection of turnip plants with cauliflower mosaic virus (CaMV) does not lead to any changes in the chromatographic behaviour or in the general properties of the enzymes, except for a slight reduction in the degree of inhibition by phosphate of DNA polymerase-β. The data are discussed in relation to the possible identify of CaMV replicase in infected plants.</abstract><cop>London</cop><pub>Oxford University Press</pub><doi>10.1093/oxfordjournals.aob.a087096</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0305-7364 |
| ispartof | Annals of botany, 1986, Vol.57 (1), p.81-89 |
| issn | 0305-7364 1095-8290 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14563134 |
| source | Oxford University Press Journals Digital Archive Legacy; JSTOR Archive Collection A-Z Listing |
| subjects | Biochemistry Biological and medical sciences Brassica rapa Brassica rapa subsp. campestris Cauliflower mosaic virus cauliflower mosaic virus replicase Caulimovirus Chromatography DNA DNA biosynthesis DNA polymerase DNA replication DNA-directed DNA polymerase enzyme activity Enzymes Fundamental and applied biological sciences. Psychology Pathology. Damages, economic importance Phosphates Phytopathology. Animal pests. Plant and forest protection Plant cells Plant viruses and viroids Plants Turnip Turnips |
| title | DNA polymerase activities in healthy and cauliflower mosaic virus-infected turnip (Brassica rapa) plants |
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