Wound Tissue Malondialdehyde and Glutathione Levels in Leptin Treated Healthy and Diabetic Rats
[LANGUAGE= "English"] Objectives: We aimed to investigate the effects of leptin on wound tissue malondialdehyde and reduced glutathione levels in Streptozotocin-induced diabetic and healthy rats.Patients and Methods: Experiments were performed on 28 male Wistar rats. Animals were divided a...
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description | [LANGUAGE= "English"] Objectives: We aimed to investigate the effects of leptin on wound tissue malondialdehyde and reduced glutathione levels in Streptozotocin-induced diabetic and healthy rats.Patients and Methods: Experiments were performed on 28 male Wistar rats. Animals were divided as healthy control (n = 14) and Streptozotocin -induced diabetic (n = 14) rats. Diabetes was induced by injection of Streptozotocin (intraperitoneally, 55 mg/kg bw). On the 7th day after administration of Streptozotocin, six full-thickness excisional wounds were made under anesthesia in all animals. Then, both healthy and diabetic animals were divided into systemic leptin (intraperitoneally; 0.1mg/kg/day, for 5 days) and vehicle (Phosphate buffered saline (PBS)) subgroups. For topical administrations, wounds of all rats were covered with 5 µg leptin (in 20 µl PBS, for 5 days- right side wounds) and vehicle (left side wounds). At the end of these administrations, the animals were sacrificed and MDA and GSH analysis were made on wound tissues.Results: High MDA and low GSH levels were observed in diabetic control wounds than in healthy control wounds. Topical treatment of normal wounds and systemic and topical co- treatment of diabetic wounds with leptin decreased malondialdehyde levels of wound tissue.Conclusion: Wound tissue oxidative damage might diminish via leptin in healthy and diabetic rats. |
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Animals were divided as healthy control (n = 14) and Streptozotocin -induced diabetic (n = 14) rats. Diabetes was induced by injection of Streptozotocin (intraperitoneally, 55 mg/kg bw). On the 7th day after administration of Streptozotocin, six full-thickness excisional wounds were made under anesthesia in all animals. Then, both healthy and diabetic animals were divided into systemic leptin (intraperitoneally; 0.1mg/kg/day, for 5 days) and vehicle (Phosphate buffered saline (PBS)) subgroups. For topical administrations, wounds of all rats were covered with 5 µg leptin (in 20 µl PBS, for 5 days- right side wounds) and vehicle (left side wounds). At the end of these administrations, the animals were sacrificed and MDA and GSH analysis were made on wound tissues.Results: High MDA and low GSH levels were observed in diabetic control wounds than in healthy control wounds. Topical treatment of normal wounds and systemic and topical co- treatment of diabetic wounds with leptin decreased malondialdehyde levels of wound tissue.Conclusion: Wound tissue oxidative damage might diminish via leptin in healthy and diabetic rats.</description><identifier>EISSN: 2980-2156</identifier><language>tur</language><publisher>Istanbul: Kare Publishing</publisher><subject>Diabetes</subject><ispartof>Journal of Clinical Practice & Research, 2010-01, Vol.32 (3), p.161</ispartof><rights>2010. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). 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Animals were divided as healthy control (n = 14) and Streptozotocin -induced diabetic (n = 14) rats. Diabetes was induced by injection of Streptozotocin (intraperitoneally, 55 mg/kg bw). On the 7th day after administration of Streptozotocin, six full-thickness excisional wounds were made under anesthesia in all animals. Then, both healthy and diabetic animals were divided into systemic leptin (intraperitoneally; 0.1mg/kg/day, for 5 days) and vehicle (Phosphate buffered saline (PBS)) subgroups. For topical administrations, wounds of all rats were covered with 5 µg leptin (in 20 µl PBS, for 5 days- right side wounds) and vehicle (left side wounds). At the end of these administrations, the animals were sacrificed and MDA and GSH analysis were made on wound tissues.Results: High MDA and low GSH levels were observed in diabetic control wounds than in healthy control wounds. Topical treatment of normal wounds and systemic and topical co- treatment of diabetic wounds with leptin decreased malondialdehyde levels of wound tissue.Conclusion: Wound tissue oxidative damage might diminish via leptin in healthy and diabetic rats.</description><subject>Diabetes</subject><issn>2980-2156</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNi8sKwjAUBYMgWLT_EHBdSGtbm7WvLnQjBZflaq40JSS1SYT-vUH8AFdzYObMSJTxiiVZWpQLElvbM8YynhcFryLS3ozXgjbSWo_0AspoIUEJ7CaBFII6Ke_AddJopGd8o7JU6rAGF9CMCA4FrRGU66bvYS_hjk4-6BWcXZH5E5TF-MclWR8Pza5OhtG8PFrX9saPOqg2q3jOGU_L7ea_6gOTrkRT</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Dinçer, Sibel</creator><creator>Gülen, Şebnem</creator><general>Kare Publishing</general><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20100101</creationdate><title>Wound Tissue Malondialdehyde and Glutathione Levels in Leptin Treated Healthy and Diabetic Rats</title><author>Dinçer, Sibel ; Gülen, Şebnem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_28949091673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>tur</language><creationdate>2010</creationdate><topic>Diabetes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dinçer, Sibel</creatorcontrib><creatorcontrib>Gülen, Şebnem</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of Clinical Practice & Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dinçer, Sibel</au><au>Gülen, Şebnem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wound Tissue Malondialdehyde and Glutathione Levels in Leptin Treated Healthy and Diabetic Rats</atitle><jtitle>Journal of Clinical Practice & Research</jtitle><date>2010-01-01</date><risdate>2010</risdate><volume>32</volume><issue>3</issue><spage>161</spage><pages>161-</pages><eissn>2980-2156</eissn><abstract>[LANGUAGE= "English"] Objectives: We aimed to investigate the effects of leptin on wound tissue malondialdehyde and reduced glutathione levels in Streptozotocin-induced diabetic and healthy rats.Patients and Methods: Experiments were performed on 28 male Wistar rats. Animals were divided as healthy control (n = 14) and Streptozotocin -induced diabetic (n = 14) rats. Diabetes was induced by injection of Streptozotocin (intraperitoneally, 55 mg/kg bw). On the 7th day after administration of Streptozotocin, six full-thickness excisional wounds were made under anesthesia in all animals. Then, both healthy and diabetic animals were divided into systemic leptin (intraperitoneally; 0.1mg/kg/day, for 5 days) and vehicle (Phosphate buffered saline (PBS)) subgroups. For topical administrations, wounds of all rats were covered with 5 µg leptin (in 20 µl PBS, for 5 days- right side wounds) and vehicle (left side wounds). At the end of these administrations, the animals were sacrificed and MDA and GSH analysis were made on wound tissues.Results: High MDA and low GSH levels were observed in diabetic control wounds than in healthy control wounds. Topical treatment of normal wounds and systemic and topical co- treatment of diabetic wounds with leptin decreased malondialdehyde levels of wound tissue.Conclusion: Wound tissue oxidative damage might diminish via leptin in healthy and diabetic rats.</abstract><cop>Istanbul</cop><pub>Kare Publishing</pub><oa>free_for_read</oa></addata></record> |
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subjects | Diabetes |
title | Wound Tissue Malondialdehyde and Glutathione Levels in Leptin Treated Healthy and Diabetic Rats |
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