Wound Tissue Malondialdehyde and Glutathione Levels in Leptin Treated Healthy and Diabetic Rats

[LANGUAGE= "English"] Objectives: We aimed to investigate the effects of leptin on wound tissue malondialdehyde and reduced glutathione levels in Streptozotocin-induced diabetic and healthy rats.Patients and Methods: Experiments were performed on 28 male Wistar rats. Animals were divided as healthy control (n = 14) and Streptozotocin -induced diabetic (n = 14) rats. Diabetes was induced by injection of Streptozotocin (intraperitoneally, 55 mg/kg bw). On the 7th day after administration of Streptozotocin, six full-thickness excisional wounds were made under anesthesia in all animals. Then, both healthy and diabetic animals were divided into systemic leptin (intraperitoneally; 0.1mg/kg/day, for 5 days) and vehicle (Phosphate buffered saline (PBS)) subgroups. For topical administrations, wounds of all rats were covered with 5 µg leptin (in 20 µl PBS, for 5 days- right side wounds) and vehicle (left side wounds). At the end of these administrations, the animals were sacrificed and MDA and GSH analysis were made on wound tissues.Results: High MDA and low GSH levels were observed in diabetic control wounds than in healthy control wounds. Topical treatment of normal wounds and systemic and topical co- treatment of diabetic wounds with leptin decreased malondialdehyde levels of wound tissue.Conclusion: Wound tissue oxidative damage might diminish via leptin in healthy and diabetic rats.