P-146: Genetic polymorphism of angiotensin converting enzyme (ACE) and its relationship with haptoglobin and angiotensinogen genotypes and biochemical markers of cardiovascular pathology, in adolescents

Angiotensin I converting enzyme (ACE I/D), haptoglobin (Hp 1/2) and angiotensinogen (AGT M235T) genes polymorphisms have been associated with risk of several cardiovascular conditions. We have studied, in a healthy sample of adolescents randomly selected, the relationship between those genes polymor...

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Veröffentlicht in:American journal of hypertension 2001-04, Vol.14 (S1), p.79A-79A
Hauptverfasser: Coelho, C., Guerra, A., Breitenfeld, L., Rego, C., Castro, E.M.B, Rodrigues, P., Laires, M. J., Bicho, M.
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container_end_page 79A
container_issue S1
container_start_page 79A
container_title American journal of hypertension
container_volume 14
creator Coelho, C.
Guerra, A.
Breitenfeld, L.
Rego, C.
Castro, E.M.B
Rodrigues, P.
Laires, M. J.
Bicho, M.
description Angiotensin I converting enzyme (ACE I/D), haptoglobin (Hp 1/2) and angiotensinogen (AGT M235T) genes polymorphisms have been associated with risk of several cardiovascular conditions. We have studied, in a healthy sample of adolescents randomly selected, the relationship between those genes polymorphisms and somatic characteristics, blood pressure and some biochemical markers of cardiovascular risk, mainly those related to oxidative stress. A subsample of 38 healthy adolescents, included in a larger cohort of children studied from 2 to 5 years of age, were re-evaluated at 12 to 15 years of age. ACE and AGT genotypes were evaluated, respectively, by PCR and PCR-RFLP; ACE and erythrocyte transmembrane oxido-reductase (TMR) activities by spectrophotometry; haptoglobin phenotype by PAGE; active renin by RIA; total and LDL-MDA by spectrophotometric quantification of substances that react with tiobarbituric acid (TBARS), and expressed according to total cholesterol, LDL-cholesterol and ApoB levels. On the last evaluation, no associations were found between blood pressure, lipid profile, anthropometric parameters, body composition, active renin or TMR and the ACE I/D gene polymorphism. Males had higher values for ACE activity (75.9 U/L) than females (48.1 U/L) (p
doi_str_mv 10.1016/S0895-7061(01)01755-1
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J. ; Bicho, M.</creator><creatorcontrib>Coelho, C. ; Guerra, A. ; Breitenfeld, L. ; Rego, C. ; Castro, E.M.B ; Rodrigues, P. ; Laires, M. J. ; Bicho, M.</creatorcontrib><description>Angiotensin I converting enzyme (ACE I/D), haptoglobin (Hp 1/2) and angiotensinogen (AGT M235T) genes polymorphisms have been associated with risk of several cardiovascular conditions. We have studied, in a healthy sample of adolescents randomly selected, the relationship between those genes polymorphisms and somatic characteristics, blood pressure and some biochemical markers of cardiovascular risk, mainly those related to oxidative stress. A subsample of 38 healthy adolescents, included in a larger cohort of children studied from 2 to 5 years of age, were re-evaluated at 12 to 15 years of age. ACE and AGT genotypes were evaluated, respectively, by PCR and PCR-RFLP; ACE and erythrocyte transmembrane oxido-reductase (TMR) activities by spectrophotometry; haptoglobin phenotype by PAGE; active renin by RIA; total and LDL-MDA by spectrophotometric quantification of substances that react with tiobarbituric acid (TBARS), and expressed according to total cholesterol, LDL-cholesterol and ApoB levels. On the last evaluation, no associations were found between blood pressure, lipid profile, anthropometric parameters, body composition, active renin or TMR and the ACE I/D gene polymorphism. Males had higher values for ACE activity (75.9 U/L) than females (48.1 U/L) (p&lt;0.01). Haptoglobin allele Hp1 was significantly more associated with higher ACE activities (Hp1.1 = 75.1 U/L, Hp2.2 = 48.1 U/L, p&lt;0.05), and so was the ACE D allele (II = 38.6 U/L, ID = 46.2 U/L, DD = 76.8 U/L, p&lt;0.001). ACE activity was similar between AGT genotypes. Higher levels of LDL-MDA/ApoB were observed in individuals with both ACE DD and Hp 2.2 genotypes. A higher level od LDL-MDA/ApoB was also observed in AGT MT genotype compared to MM and TT genotypes. These latter showed higher medium and diastolic blood pressure. We conclude that healthy adolescents have higher activities of ACE in ACE DD genotype carriers. Oxidative stress markers were more associated to AGT MT, ACE DD and Hp 2.2 genotypes. Furthermore, in AGT TT individuals, medium and diastolic blood pressures were higher compared to the other AGT genotypes. ACE and AGT genes might behave independently.</description><identifier>ISSN: 0895-7061</identifier><identifier>EISSN: 1941-7225</identifier><identifier>DOI: 10.1016/S0895-7061(01)01755-1</identifier><identifier>CODEN: AJHYE6</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>angiotensin I converting enzyme ; angiotensinogen ; haptoglobin</subject><ispartof>American journal of hypertension, 2001-04, Vol.14 (S1), p.79A-79A</ispartof><rights>Copyright Nature Publishing Group Apr 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Coelho, C.</creatorcontrib><creatorcontrib>Guerra, A.</creatorcontrib><creatorcontrib>Breitenfeld, L.</creatorcontrib><creatorcontrib>Rego, C.</creatorcontrib><creatorcontrib>Castro, E.M.B</creatorcontrib><creatorcontrib>Rodrigues, P.</creatorcontrib><creatorcontrib>Laires, M. J.</creatorcontrib><creatorcontrib>Bicho, M.</creatorcontrib><title>P-146: Genetic polymorphism of angiotensin converting enzyme (ACE) and its relationship with haptoglobin and angiotensinogen genotypes and biochemical markers of cardiovascular pathology, in adolescents</title><title>American journal of hypertension</title><addtitle>AJH</addtitle><description>Angiotensin I converting enzyme (ACE I/D), haptoglobin (Hp 1/2) and angiotensinogen (AGT M235T) genes polymorphisms have been associated with risk of several cardiovascular conditions. We have studied, in a healthy sample of adolescents randomly selected, the relationship between those genes polymorphisms and somatic characteristics, blood pressure and some biochemical markers of cardiovascular risk, mainly those related to oxidative stress. A subsample of 38 healthy adolescents, included in a larger cohort of children studied from 2 to 5 years of age, were re-evaluated at 12 to 15 years of age. ACE and AGT genotypes were evaluated, respectively, by PCR and PCR-RFLP; ACE and erythrocyte transmembrane oxido-reductase (TMR) activities by spectrophotometry; haptoglobin phenotype by PAGE; active renin by RIA; total and LDL-MDA by spectrophotometric quantification of substances that react with tiobarbituric acid (TBARS), and expressed according to total cholesterol, LDL-cholesterol and ApoB levels. On the last evaluation, no associations were found between blood pressure, lipid profile, anthropometric parameters, body composition, active renin or TMR and the ACE I/D gene polymorphism. Males had higher values for ACE activity (75.9 U/L) than females (48.1 U/L) (p&lt;0.01). Haptoglobin allele Hp1 was significantly more associated with higher ACE activities (Hp1.1 = 75.1 U/L, Hp2.2 = 48.1 U/L, p&lt;0.05), and so was the ACE D allele (II = 38.6 U/L, ID = 46.2 U/L, DD = 76.8 U/L, p&lt;0.001). ACE activity was similar between AGT genotypes. Higher levels of LDL-MDA/ApoB were observed in individuals with both ACE DD and Hp 2.2 genotypes. A higher level od LDL-MDA/ApoB was also observed in AGT MT genotype compared to MM and TT genotypes. These latter showed higher medium and diastolic blood pressure. We conclude that healthy adolescents have higher activities of ACE in ACE DD genotype carriers. Oxidative stress markers were more associated to AGT MT, ACE DD and Hp 2.2 genotypes. Furthermore, in AGT TT individuals, medium and diastolic blood pressures were higher compared to the other AGT genotypes. 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J.</creatorcontrib><creatorcontrib>Bicho, M.</creatorcontrib><collection>Istex</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>American journal of hypertension</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coelho, C.</au><au>Guerra, A.</au><au>Breitenfeld, L.</au><au>Rego, C.</au><au>Castro, E.M.B</au><au>Rodrigues, P.</au><au>Laires, M. J.</au><au>Bicho, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P-146: Genetic polymorphism of angiotensin converting enzyme (ACE) and its relationship with haptoglobin and angiotensinogen genotypes and biochemical markers of cardiovascular pathology, in adolescents</atitle><jtitle>American journal of hypertension</jtitle><addtitle>AJH</addtitle><date>2001-04</date><risdate>2001</risdate><volume>14</volume><issue>S1</issue><spage>79A</spage><epage>79A</epage><pages>79A-79A</pages><issn>0895-7061</issn><eissn>1941-7225</eissn><coden>AJHYE6</coden><abstract>Angiotensin I converting enzyme (ACE I/D), haptoglobin (Hp 1/2) and angiotensinogen (AGT M235T) genes polymorphisms have been associated with risk of several cardiovascular conditions. We have studied, in a healthy sample of adolescents randomly selected, the relationship between those genes polymorphisms and somatic characteristics, blood pressure and some biochemical markers of cardiovascular risk, mainly those related to oxidative stress. A subsample of 38 healthy adolescents, included in a larger cohort of children studied from 2 to 5 years of age, were re-evaluated at 12 to 15 years of age. ACE and AGT genotypes were evaluated, respectively, by PCR and PCR-RFLP; ACE and erythrocyte transmembrane oxido-reductase (TMR) activities by spectrophotometry; haptoglobin phenotype by PAGE; active renin by RIA; total and LDL-MDA by spectrophotometric quantification of substances that react with tiobarbituric acid (TBARS), and expressed according to total cholesterol, LDL-cholesterol and ApoB levels. On the last evaluation, no associations were found between blood pressure, lipid profile, anthropometric parameters, body composition, active renin or TMR and the ACE I/D gene polymorphism. Males had higher values for ACE activity (75.9 U/L) than females (48.1 U/L) (p&lt;0.01). Haptoglobin allele Hp1 was significantly more associated with higher ACE activities (Hp1.1 = 75.1 U/L, Hp2.2 = 48.1 U/L, p&lt;0.05), and so was the ACE D allele (II = 38.6 U/L, ID = 46.2 U/L, DD = 76.8 U/L, p&lt;0.001). ACE activity was similar between AGT genotypes. Higher levels of LDL-MDA/ApoB were observed in individuals with both ACE DD and Hp 2.2 genotypes. A higher level od LDL-MDA/ApoB was also observed in AGT MT genotype compared to MM and TT genotypes. These latter showed higher medium and diastolic blood pressure. We conclude that healthy adolescents have higher activities of ACE in ACE DD genotype carriers. Oxidative stress markers were more associated to AGT MT, ACE DD and Hp 2.2 genotypes. Furthermore, in AGT TT individuals, medium and diastolic blood pressures were higher compared to the other AGT genotypes. ACE and AGT genes might behave independently.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><doi>10.1016/S0895-7061(01)01755-1</doi></addata></record>
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source Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection
subjects angiotensin I converting enzyme
angiotensinogen
haptoglobin
title P-146: Genetic polymorphism of angiotensin converting enzyme (ACE) and its relationship with haptoglobin and angiotensinogen genotypes and biochemical markers of cardiovascular pathology, in adolescents
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