P-146: Genetic polymorphism of angiotensin converting enzyme (ACE) and its relationship with haptoglobin and angiotensinogen genotypes and biochemical markers of cardiovascular pathology, in adolescents

Angiotensin I converting enzyme (ACE I/D), haptoglobin (Hp 1/2) and angiotensinogen (AGT M235T) genes polymorphisms have been associated with risk of several cardiovascular conditions. We have studied, in a healthy sample of adolescents randomly selected, the relationship between those genes polymorphisms and somatic characteristics, blood pressure and some biochemical markers of cardiovascular risk, mainly those related to oxidative stress. A subsample of 38 healthy adolescents, included in a larger cohort of children studied from 2 to 5 years of age, were re-evaluated at 12 to 15 years of age. ACE and AGT genotypes were evaluated, respectively, by PCR and PCR-RFLP; ACE and erythrocyte transmembrane oxido-reductase (TMR) activities by spectrophotometry; haptoglobin phenotype by PAGE; active renin by RIA; total and LDL-MDA by spectrophotometric quantification of substances that react with tiobarbituric acid (TBARS), and expressed according to total cholesterol, LDL-cholesterol and ApoB levels. On the last evaluation, no associations were found between blood pressure, lipid profile, anthropometric parameters, body composition, active renin or TMR and the ACE I/D gene polymorphism. Males had higher values for ACE activity (75.9 U/L) than females (48.1 U/L) (p