Identification and Quantification of Glucosinolates and Phenolics in a Large Panel of Brassica napus Highlight Valuable Genetic Resources for Chemical Ecology and Breeding

Glucosinolate (GLS) and phenolic contents in Brassicaceae contribute to biotic and abiotic stress responses. Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassica germplasm. This work investigates the diversit...

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Veröffentlicht in:Journal of agricultural and food chemistry 2022-04, Vol.70 (16), p.5245-5261
Hauptverfasser: Missinou, Anani Amegan, Ferreira de Carvalho, Julie, Marnet, Nathalie, Delhaye, Thomas, Hamzaoui, Oumayma, Abdel Sayed, David, Guitton, Yann, Lebreton, Lionel, Langrume, Christophe, Laperche, Anne, Delourme, Régine, Manzanares-Dauleux, Maria J, Bouchereau, Alain, Gravot, Antoine
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Sprache:eng
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Zusammenfassung:Glucosinolate (GLS) and phenolic contents in Brassicaceae contribute to biotic and abiotic stress responses. Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassica germplasm. This work investigates the diversity of specialized metabolites in 281 accessions of B. napus. First, an LC-HRMS2-based approach allowed the annotation of 32 phenolics and 36 GLSs, revealing 13 branched and linear alkyl-GLSs and 4 isomers of hydroxyphenylalkyl-GLSs, many of which have been rarely reported in Brassica. Then, quantitative UPLC-UV-MS-based profiling was performed in leaves and roots for the whole panel. This revealed striking variations in the content of 1-methylpropyl-GLS (glucocochlearin) and a large variation of tetra- and penta-glucosyl kaempferol derivatives among accessions. It also highlighted two main chemotypes related to sinapoyl-O-hexoside and kaempferol-O-trihexoside contents. By offering an unprecedented overview of the phytochemical diversity in B. napus, this work provides a useful resource for chemical ecology and breeding.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.1c08118