Identification and Quantification of Glucosinolates and Phenolics in a Large Panel of Brassica napus Highlight Valuable Genetic Resources for Chemical Ecology and Breeding
Glucosinolate (GLS) and phenolic contents in Brassicaceae contribute to biotic and abiotic stress responses. Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassica germplasm. This work investigates the diversit...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2022-04, Vol.70 (16), p.5245-5261 |
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| creator | Missinou, Anani Amegan Ferreira de Carvalho, Julie Marnet, Nathalie Delhaye, Thomas Hamzaoui, Oumayma Abdel Sayed, David Guitton, Yann Lebreton, Lionel Langrume, Christophe Laperche, Anne Delourme, Régine Manzanares-Dauleux, Maria J Bouchereau, Alain Gravot, Antoine |
| description | Glucosinolate (GLS) and phenolic contents in Brassicaceae contribute to biotic and abiotic stress responses. Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassica germplasm. This work investigates the diversity of specialized metabolites in 281 accessions of B. napus. First, an LC-HRMS2-based approach allowed the annotation of 32 phenolics and 36 GLSs, revealing 13 branched and linear alkyl-GLSs and 4 isomers of hydroxyphenylalkyl-GLSs, many of which have been rarely reported in Brassica. Then, quantitative UPLC-UV-MS-based profiling was performed in leaves and roots for the whole panel. This revealed striking variations in the content of 1-methylpropyl-GLS (glucocochlearin) and a large variation of tetra- and penta-glucosyl kaempferol derivatives among accessions. It also highlighted two main chemotypes related to sinapoyl-O-hexoside and kaempferol-O-trihexoside contents. By offering an unprecedented overview of the phytochemical diversity in B. napus, this work provides a useful resource for chemical ecology and breeding. |
| doi_str_mv | 10.1021/acs.jafc.1c08118 |
| format | Article |
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Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassica germplasm. This work investigates the diversity of specialized metabolites in 281 accessions of B. napus. First, an LC-HRMS2-based approach allowed the annotation of 32 phenolics and 36 GLSs, revealing 13 branched and linear alkyl-GLSs and 4 isomers of hydroxyphenylalkyl-GLSs, many of which have been rarely reported in Brassica. Then, quantitative UPLC-UV-MS-based profiling was performed in leaves and roots for the whole panel. This revealed striking variations in the content of 1-methylpropyl-GLS (glucocochlearin) and a large variation of tetra- and penta-glucosyl kaempferol derivatives among accessions. It also highlighted two main chemotypes related to sinapoyl-O-hexoside and kaempferol-O-trihexoside contents. By offering an unprecedented overview of the phytochemical diversity in B. napus, this work provides a useful resource for chemical ecology and breeding.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.1c08118</identifier><identifier>PMID: 35420430</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biodiversity and Ecology ; Brassica - metabolism ; Brassica napus - metabolism ; Breeding ; Chemical Sciences ; Cheminformatics ; Environmental Sciences ; Glucosinolates - metabolism ; Kaempferols ; Omics Technologies Applied to Agriculture and Food ; Phenols</subject><ispartof>Journal of agricultural and food chemistry, 2022-04, Vol.70 (16), p.5245-5261</ispartof><rights>2022 American Chemical Society</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a370t-50eede6052c43cbe5913f565f898d1aac50dbce1b31caaefaf311e223b2fd3f73</citedby><cites>FETCH-LOGICAL-a370t-50eede6052c43cbe5913f565f898d1aac50dbce1b31caaefaf311e223b2fd3f73</cites><orcidid>0000-0001-9125-1494 ; 0000-0001-6200-3344 ; 0000-0002-6452-0393 ; 0000-0002-2473-3773 ; 0000-0002-4479-0636 ; 0000-0003-1759-5269 ; 0000-0002-4930-8407</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.1c08118$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.1c08118$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35420430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-03700611$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Missinou, Anani Amegan</creatorcontrib><creatorcontrib>Ferreira de Carvalho, Julie</creatorcontrib><creatorcontrib>Marnet, Nathalie</creatorcontrib><creatorcontrib>Delhaye, Thomas</creatorcontrib><creatorcontrib>Hamzaoui, Oumayma</creatorcontrib><creatorcontrib>Abdel Sayed, David</creatorcontrib><creatorcontrib>Guitton, Yann</creatorcontrib><creatorcontrib>Lebreton, Lionel</creatorcontrib><creatorcontrib>Langrume, Christophe</creatorcontrib><creatorcontrib>Laperche, Anne</creatorcontrib><creatorcontrib>Delourme, Régine</creatorcontrib><creatorcontrib>Manzanares-Dauleux, Maria J</creatorcontrib><creatorcontrib>Bouchereau, Alain</creatorcontrib><creatorcontrib>Gravot, Antoine</creatorcontrib><title>Identification and Quantification of Glucosinolates and Phenolics in a Large Panel of Brassica napus Highlight Valuable Genetic Resources for Chemical Ecology and Breeding</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Glucosinolate (GLS) and phenolic contents in Brassicaceae contribute to biotic and abiotic stress responses. Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassica germplasm. This work investigates the diversity of specialized metabolites in 281 accessions of B. napus. First, an LC-HRMS2-based approach allowed the annotation of 32 phenolics and 36 GLSs, revealing 13 branched and linear alkyl-GLSs and 4 isomers of hydroxyphenylalkyl-GLSs, many of which have been rarely reported in Brassica. Then, quantitative UPLC-UV-MS-based profiling was performed in leaves and roots for the whole panel. This revealed striking variations in the content of 1-methylpropyl-GLS (glucocochlearin) and a large variation of tetra- and penta-glucosyl kaempferol derivatives among accessions. 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Ferreira de Carvalho, Julie ; Marnet, Nathalie ; Delhaye, Thomas ; Hamzaoui, Oumayma ; Abdel Sayed, David ; Guitton, Yann ; Lebreton, Lionel ; Langrume, Christophe ; Laperche, Anne ; Delourme, Régine ; Manzanares-Dauleux, Maria J ; Bouchereau, Alain ; Gravot, Antoine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a370t-50eede6052c43cbe5913f565f898d1aac50dbce1b31caaefaf311e223b2fd3f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biodiversity and Ecology</topic><topic>Brassica - metabolism</topic><topic>Brassica napus - metabolism</topic><topic>Breeding</topic><topic>Chemical Sciences</topic><topic>Cheminformatics</topic><topic>Environmental Sciences</topic><topic>Glucosinolates - metabolism</topic><topic>Kaempferols</topic><topic>Omics Technologies Applied to Agriculture and Food</topic><topic>Phenols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Missinou, Anani Amegan</creatorcontrib><creatorcontrib>Ferreira de Carvalho, Julie</creatorcontrib><creatorcontrib>Marnet, Nathalie</creatorcontrib><creatorcontrib>Delhaye, Thomas</creatorcontrib><creatorcontrib>Hamzaoui, Oumayma</creatorcontrib><creatorcontrib>Abdel Sayed, David</creatorcontrib><creatorcontrib>Guitton, Yann</creatorcontrib><creatorcontrib>Lebreton, Lionel</creatorcontrib><creatorcontrib>Langrume, Christophe</creatorcontrib><creatorcontrib>Laperche, Anne</creatorcontrib><creatorcontrib>Delourme, Régine</creatorcontrib><creatorcontrib>Manzanares-Dauleux, Maria J</creatorcontrib><creatorcontrib>Bouchereau, Alain</creatorcontrib><creatorcontrib>Gravot, Antoine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Missinou, Anani Amegan</au><au>Ferreira de Carvalho, Julie</au><au>Marnet, Nathalie</au><au>Delhaye, Thomas</au><au>Hamzaoui, Oumayma</au><au>Abdel Sayed, David</au><au>Guitton, Yann</au><au>Lebreton, Lionel</au><au>Langrume, Christophe</au><au>Laperche, Anne</au><au>Delourme, Régine</au><au>Manzanares-Dauleux, Maria J</au><au>Bouchereau, Alain</au><au>Gravot, Antoine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and Quantification of Glucosinolates and Phenolics in a Large Panel of Brassica napus Highlight Valuable Genetic Resources for Chemical Ecology and Breeding</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2022-04-27</date><risdate>2022</risdate><volume>70</volume><issue>16</issue><spage>5245</spage><epage>5261</epage><pages>5245-5261</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Glucosinolate (GLS) and phenolic contents in Brassicaceae contribute to biotic and abiotic stress responses. Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassica germplasm. This work investigates the diversity of specialized metabolites in 281 accessions of B. napus. First, an LC-HRMS2-based approach allowed the annotation of 32 phenolics and 36 GLSs, revealing 13 branched and linear alkyl-GLSs and 4 isomers of hydroxyphenylalkyl-GLSs, many of which have been rarely reported in Brassica. Then, quantitative UPLC-UV-MS-based profiling was performed in leaves and roots for the whole panel. This revealed striking variations in the content of 1-methylpropyl-GLS (glucocochlearin) and a large variation of tetra- and penta-glucosyl kaempferol derivatives among accessions. It also highlighted two main chemotypes related to sinapoyl-O-hexoside and kaempferol-O-trihexoside contents. 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| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2022-04, Vol.70 (16), p.5245-5261 |
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| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Biodiversity and Ecology Brassica - metabolism Brassica napus - metabolism Breeding Chemical Sciences Cheminformatics Environmental Sciences Glucosinolates - metabolism Kaempferols Omics Technologies Applied to Agriculture and Food Phenols |
| title | Identification and Quantification of Glucosinolates and Phenolics in a Large Panel of Brassica napus Highlight Valuable Genetic Resources for Chemical Ecology and Breeding |
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