UNIVERSAL FLUORESCENT NANOPROBES FOR ONE-STEP ISOTHERMAL NUCLEIC ACID AMPLIFICATION ASSAY

The present invention provides a universal fluorescent probing system and method for result readout of loop-mediated isothermal amplification (LAMP) or reverse transcription-loop-mediated isothermal amplification (RT-LAMP) in a closed-tube manner using at least three different types of universal qua...

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Bibliographische Detailangaben
Hauptverfasser: WANG, Shiyao, LEE, Ming Hung Thomas, YIP, Shea Ping, QIN, Ailin
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The present invention provides a universal fluorescent probing system and method for result readout of loop-mediated isothermal amplification (LAMP) or reverse transcription-loop-mediated isothermal amplification (RT-LAMP) in a closed-tube manner using at least three different types of universal quantum dot probes, including sulfonate-, carboxyl-, and amine-modified quantum dots (QDs). Detection mechanism of the present system and method relies on a co-precipitation of the modified QDs with magnesium pyrophosphate crystals (one of the positive LAMP/RT-LAMP reaction products) formed during the reaction. The present system and method are advantageous over the existing technologies in terms of simple preparation, low cost, as well as excellent universality, specificity and sensitivity.