UNIVERSAL FLUORESCENT NANOPROBES FOR ONE-STEP ISOTHERMAL NUCLEIC ACID AMPLIFICATION ASSAY
The present invention provides a universal fluorescent probing system and method for result readout of loop-mediated isothermal amplification (LAMP) or reverse transcription-loop-mediated isothermal amplification (RT-LAMP) in a closed-tube manner using at least three different types of universal qua...
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| creator | WANG, Shiyao LEE, Ming Hung Thomas YIP, Shea Ping QIN, Ailin |
| description | The present invention provides a universal fluorescent probing system and method for result readout of loop-mediated isothermal amplification (LAMP) or reverse transcription-loop-mediated isothermal amplification (RT-LAMP) in a closed-tube manner using at least three different types of universal quantum dot probes, including sulfonate-, carboxyl-, and amine-modified quantum dots (QDs). Detection mechanism of the present system and method relies on a co-precipitation of the modified QDs with magnesium pyrophosphate crystals (one of the positive LAMP/RT-LAMP reaction products) formed during the reaction. The present system and method are advantageous over the existing technologies in terms of simple preparation, low cost, as well as excellent universality, specificity and sensitivity. |
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Detection mechanism of the present system and method relies on a co-precipitation of the modified QDs with magnesium pyrophosphate crystals (one of the positive LAMP/RT-LAMP reaction products) formed during the reaction. 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Detection mechanism of the present system and method relies on a co-precipitation of the modified QDs with magnesium pyrophosphate crystals (one of the positive LAMP/RT-LAMP reaction products) formed during the reaction. The present system and method are advantageous over the existing technologies in terms of simple preparation, low cost, as well as excellent universality, specificity and sensitivity.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | UNIVERSAL FLUORESCENT NANOPROBES FOR ONE-STEP ISOTHERMAL NUCLEIC ACID AMPLIFICATION ASSAY |
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