Bovine pasteurella multocida capsular type A ELISA antibody detection kit and preparation method and application thereof

The invention belongs to the technical field of pathogenic bacteria detection, and particularly relates to a bovine pasteurella multocida capsular type A ELISA antibody detection kit and a preparation method and application thereof. The method comprises the following steps: integrating a gene with a...

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Hauptverfasser: GAO YANG, ZHAO YANGYANG, ZHAO ZONGLING, YANG YUANLI, QIN SHIRONG, LAI MAOLIN, RAN ZHIGUANG, LYU FENGBO, YANG JIE, LI CHAOYANG, FU GANG, WANG WEIMIN
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creator GAO YANG
ZHAO YANGYANG
ZHAO ZONGLING
YANG YUANLI
QIN SHIRONG
LAI MAOLIN
RAN ZHIGUANG
LYU FENGBO
YANG JIE
LI CHAOYANG
FU GANG
WANG WEIMIN
description The invention belongs to the technical field of pathogenic bacteria detection, and particularly relates to a bovine pasteurella multocida capsular type A ELISA antibody detection kit and a preparation method and application thereof. The method comprises the following steps: integrating a gene with a sequence as shown in SEQ ID NO.1 onto an expression vector and transferring into host bacteria; inducing host bacteria to express, and purifying to obtain a PmA153 recombinant antigen protein with a sequence as shown in SEQ ID NO.2; the PmA153 is coated on a solid-phase carrier, so that the detection of the bovine pasteurella multocida capsular A type antibody in a sample can be realized through an enzyme-linked immunosorbent assay method, and the purpose of diagnosis is realized. The kit provided by the invention does not use pasteurella multocida as an antigen source, has good biological safety and production cheapness, has good specificity on bovine pasteurella multocida capsular A-type antibody detection, can
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
title Bovine pasteurella multocida capsular type A ELISA antibody detection kit and preparation method and application thereof
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