Bovine pasteurella multocida capsular type A ELISA antibody detection kit and preparation method and application thereof

The invention belongs to the technical field of pathogenic bacteria detection, and particularly relates to a bovine pasteurella multocida capsular type A ELISA antibody detection kit and a preparation method and application thereof. The method comprises the following steps: integrating a gene with a sequence as shown in SEQ ID NO.1 onto an expression vector and transferring into host bacteria; inducing host bacteria to express, and purifying to obtain a PmA153 recombinant antigen protein with a sequence as shown in SEQ ID NO.2; the PmA153 is coated on a solid-phase carrier, so that the detection of the bovine pasteurella multocida capsular A type antibody in a sample can be realized through an enzyme-linked immunosorbent assay method, and the purpose of diagnosis is realized. The kit provided by the invention does not use pasteurella multocida as an antigen source, has good biological safety and production cheapness, has good specificity on bovine pasteurella multocida capsular A-type antibody detection, can