Biopolymer sequencing method

The present disclosure provides devices, systems, and methods related to biopolymer sequencing. In particular, the present disclosure relates to methods of sequencing polynucleotides using a bioelectronic device that obtains a bioelectronic signature (e.g., current amplitude level) of polymerase act...

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1. Verfasser: LYNDERSAY SEAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present disclosure provides devices, systems, and methods related to biopolymer sequencing. In particular, the present disclosure relates to methods of sequencing polynucleotides using a bioelectronic device that obtains a bioelectronic signature (e.g., current amplitude level) of polymerase activity based on current fluctuations when complementary nucleotide polyphosphate monomers (e.g., with a unique charge) are incorporated into a template polynucleotide. 本公开提供了与生物聚合物测序相关的装置、系统和方法。具体而言,本公开涉及使用生物电子装置对多核苷酸进行测序的方法,所述生物电子装置基于当(例如具有独特电荷的)互补核苷酸多磷酸单体被掺入到模板多核苷酸中时的电流波动获得聚合酶活性的生物电子签名(例如电流幅度水平)。