Application of plasma miR-15b as molecular marker in sperm generation and functional evaluation

The invention provides application of a plasma miR-15b as a molecular marker in sperm generation and functional evaluation. MiRNA in the plasma of a testee is extracted and subjected to reverse transcription, a template cDNA obtained is subjected to real-time fluorescent quantitative PCR (polymerase chain reaction), and an expression of the miR-15b in the plasma is compared to that of a normal plasma sample. The miRNA miR-15b playing an important role in the process of sperm generation is discovered, the expressional differences of the miR-15b in male aspermatism and severe oligozoospermia is discovered, and evident changes in the expression of the miR-15 in the plasma can be detected; the miR-15 is suitable for laboratory detection methods of evaluating sperm quality and function, and detection and clinical consultation are provided for male patients with infertility caused by unknown clinical reasons.