Application of plasma miR-15b as molecular marker in sperm generation and functional evaluation
The invention provides application of a plasma miR-15b as a molecular marker in sperm generation and functional evaluation. MiRNA in the plasma of a testee is extracted and subjected to reverse transcription, a template cDNA obtained is subjected to real-time fluorescent quantitative PCR (polymerase...
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| creator | LIAO HUIJUAN CHEN YAN SUN HUAQIN YANG MING XU WENMING YUE HUANXUN |
| description | The invention provides application of a plasma miR-15b as a molecular marker in sperm generation and functional evaluation. MiRNA in the plasma of a testee is extracted and subjected to reverse transcription, a template cDNA obtained is subjected to real-time fluorescent quantitative PCR (polymerase chain reaction), and an expression of the miR-15b in the plasma is compared to that of a normal plasma sample. The miRNA miR-15b playing an important role in the process of sperm generation is discovered, the expressional differences of the miR-15b in male aspermatism and severe oligozoospermia is discovered, and evident changes in the expression of the miR-15 in the plasma can be detected; the miR-15 is suitable for laboratory detection methods of evaluating sperm quality and function, and detection and clinical consultation are provided for male patients with infertility caused by unknown clinical reasons. |
| format | Patent |
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MiRNA in the plasma of a testee is extracted and subjected to reverse transcription, a template cDNA obtained is subjected to real-time fluorescent quantitative PCR (polymerase chain reaction), and an expression of the miR-15b in the plasma is compared to that of a normal plasma sample. The miRNA miR-15b playing an important role in the process of sperm generation is discovered, the expressional differences of the miR-15b in male aspermatism and severe oligozoospermia is discovered, and evident changes in the expression of the miR-15 in the plasma can be detected; the miR-15 is suitable for laboratory detection methods of evaluating sperm quality and function, and detection and clinical consultation are provided for male patients with infertility caused by unknown clinical reasons.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150819&DB=EPODOC&CC=CN&NR=104846086A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150819&DB=EPODOC&CC=CN&NR=104846086A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIAO HUIJUAN</creatorcontrib><creatorcontrib>CHEN YAN</creatorcontrib><creatorcontrib>SUN HUAQIN</creatorcontrib><creatorcontrib>YANG MING</creatorcontrib><creatorcontrib>XU WENMING</creatorcontrib><creatorcontrib>YUE HUANXUN</creatorcontrib><title>Application of plasma miR-15b as molecular marker in sperm generation and functional evaluation</title><description>The invention provides application of a plasma miR-15b as a molecular marker in sperm generation and functional evaluation. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Application of plasma miR-15b as molecular marker in sperm generation and functional evaluation |
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