Liver stem cells methods and protocols

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Veröffentlicht: New York, NY [u.a.] Humana Press, Springer 2012
Schriftenreihe:Methods in molecular biology 826
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500 |a Purification and culture of fetal mouse hepatoblasts that are precursors of mature hepatocytes and biliary epithelial cells / Nobuyoshi Shiojiri and Miho Nitou -- Clinical uses of liver stem cells Clinical uses of liver stem cells / Yock Young Dan -- Identification and isolation of adult liver stem/progenitor cells / Minoru Tanaka and Atsushi Miyajima -- Isolation and purification method of mouse fetal hepatoblasts / Luc Gailhouste -- Isolation of hepatic progenitor cells from the galactosamine-treated rat liver / Norihisa Ichinohe, Junko Kon, and Toshihiro Mitaka -- Purification of adipose tissue mesenchymal stem cells and differentiation toward hepatic-like cells / Agnieszka Banas -- Development of immortalized hepatocyte-like cells from hMSCs / Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, and Suradej Hongeng -- Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages / Shohei Wakao ... [et al.] --  
500 |a Generation and hepatic differentiation of human iPS cells / Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya -- Efficient hepatic differentiation from human iPS cells by gene transfer / Kenji Kawabata, Mitsuru Inamura, and Hiroyuki Mizuguchi -- "Tet-on" system toward hepatic differentiation of human mesenchymal stem cells by hepatocyte nuclear factor / Goshi Shiota and Yoko Yoshida -- SAMe and HuR in liver physiology / Laura Gomez-Santos ... [et al.] -- Transdifferentiation of mature hepatocytes into bile duct/ductule cells within a collagen gel matrix / Yuji Nishikawa -- Identification of cancer stem cell-related microRNAs in hepatocellular carcinoma / Junfang Ji and Xin Wei Wang -- Intravenous human mesenchymal stem cells transplantation in NOD/SCID mice preserve liver integrity of irradiation damage / Moubarak Mouiseddine ... [et al.] -- Engineering of implantable liver tissues / Yasuyuki Sakai ... [et al.] --  
500 |a Mesenchymal stem cell therapy on murine model of nonalcoholic steatohepatitis / Yoshio Sakai and Shuichi Kaneko. 
650 4 |a Liver cells / Laboratory manuals 
650 4 |a Stem cells / Laboratory manuals 
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Datensatz im Suchindex

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adam_text Contents Preface .......................................................... ρ Contributors. ..................................................... ix Part I Several Hepatic Progenitor Cells 1 Purification and Culture of Fetal Mouse Hepatoblasts that Are Precursors of Mature Hepatocytes and Biliary Epithelial Cells ..................... 3 Nobuyosbi Shiojiri and Mibo Nitou 2 Clinical Uses of liver Stem Cells ................................... 11 Tock Young Dan 3 Identification and Isolation of Adult Liver Stem/Progenitor Cells .......... 25 Minoru Tuňáka and Atsushi Miyajima 4 Isolation and Purification Method of Mouse Fetal Hepatoblasts ........... 33 Luc Gaitbouste 5 Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver ..................................................... 49 Noribisa Ichinohe, Junko Kon, and Toshibiro Мішка Part II Hepatic Differentiation From Stem Cells 6 Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells ........................ 61 Agnieszka Banas 7 Development of Immortalized Hepatocyte-Iike Cells from hMSCs ......... 73 Adisak Wongkajomsilp, KbanitSa-ngiamsuntorn, and Suradej Hongeng 8 Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and Their Application to liver Damages ................ 89 Shobei Wakao, Masaaki Kitada, Tasumasa Kuroda, and Mari Dezawa 9 Generation and Hepatic Differentiation of Human iPS Cells .............. 103 Tetsuya Isbikawa, Keitaro Hagiwara, and Takabiro Ocbiya 10 Efficient Hepatic Differentiation from Human iPS Cells by Gene Transfer ............................................... 115 Kenji Kawabata, Mitsuru Inamura, and Hiroyuki Mizugucbi VII viii Contents 11 Tet-On System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor ............................ 125 Goshi Shiota and Toko Toshida 12 SAMe and HuRin Liver Physiology ................................ 133 Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato, and Maria Luz Martínez-Chantar Part III BD Formation From Stem Cells 13 Transdifferentiation of Mature Hepatocytes into Bile Duct/ductule Cells Within a Collagen Gel Matrix ................................. 153 Tuji Nishikawa Part IV Liver Stem Cells and Hepatocarcinogenesis 14 Identification of Cancer Stem Cell-Related MkroRNAs in Hepatocellular Carcinoma ...................................... 163 Junfangji and Xin Wei Wang Part V Application of Liver Stem Cells for Cell Therapy 15 Intravenous Human Mesenchymal Stem Cells Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage ....... 179 Moubarak Mouiseddine, Sabine François, Maâmar Souidi, and Alain Chapel 16 Engineering of Implantable Liver Tissues ............................ 189 Tasuyuki Sakai, M. Nishikawa, F. Bvenou, M. Hámon, H. Huang, K.P. Montagne, N. Kojima, T. Fujii, and T. Niino 17 Mesenchymal Stem Cell Therapy on Murine Model of Nonalcoholic Steatohepatitis ................................................. 217 Tosbio Sakai and Shuichi Kaneko Index ........................................................... 225 Methods in Molecular Biology 826 John M. Walker, Series Editor Takahiro Ochiya Editor Liver Stem Cells Methods and Protocols Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchy- mal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stern cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Lhvr Stem Cells: Metlwds and Pro¬ tocols, expert researchers focus on several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation troni stem cells, liver stem cells and hepatocarcinogenesi, and application ofliver stem cells for cell therapy. These topics shed light on stem cell technol¬ ogy which may lead to the development of effective clinical modalities for human liver diseases. Written in the highh- successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by- step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Liver Stem Cells: Methods and Protocols seeks to aid scientists in the further study of preclinical and clinical investigations that explore the therapeutic potential of stern cells in repair ot liver injuries.
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language English
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publishDate 2012
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publisher Humana Press, Springer
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series Methods in molecular biology
series2 Methods in molecular biology
Springer protocols
spellingShingle Liver stem cells methods and protocols
Methods in molecular biology
Liver cells / Laboratory manuals
Stem cells / Laboratory manuals
Hepatocytes / Laboratory Manuals
Stem Cells / Laboratory Manuals
Stammzelle (DE-588)4665329-6 gnd
Leber (DE-588)4034933-0 gnd
Labortechnik (DE-588)4123602-6 gnd
subject_GND (DE-588)4665329-6
(DE-588)4034933-0
(DE-588)4123602-6
title Liver stem cells methods and protocols
title_auth Liver stem cells methods and protocols
title_exact_search Liver stem cells methods and protocols
title_full Liver stem cells methods and protocols ed. by Takahiro Ochiya
title_fullStr Liver stem cells methods and protocols ed. by Takahiro Ochiya
title_full_unstemmed Liver stem cells methods and protocols ed. by Takahiro Ochiya
title_short Liver stem cells
title_sort liver stem cells methods and protocols
title_sub methods and protocols
topic Liver cells / Laboratory manuals
Stem cells / Laboratory manuals
Hepatocytes / Laboratory Manuals
Stem Cells / Laboratory Manuals
Stammzelle (DE-588)4665329-6 gnd
Leber (DE-588)4034933-0 gnd
Labortechnik (DE-588)4123602-6 gnd
topic_facet Liver cells / Laboratory manuals
Stem cells / Laboratory manuals
Hepatocytes / Laboratory Manuals
Stem Cells / Laboratory Manuals
Stammzelle
Leber
Labortechnik
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